Demand for duck eggs and meat recently increased along with the increasing interest of the community to consume duck eggs and meat. Due to that reason researcher try to find the best way to enhance the reproductive ability of livestock., which is in this research by laser puncture shot. Eighteen ducks were allocated into three groups, the control group, the first treatment group with laserpuncture shoot dose of 0.2 Joule ( J), and the second treatment group with laser puncture shoot dose of 0.4 J. Data were analyzed with ANOVA and further tested by Duncan multiple range test. Research result showed that laser puncture shoot with a dose of 0.2 J and 0.4 J decreased the number of Sertoli and Leydig cells.
This study aims to determine the effect of laser puncture shooting on the reproductive acupuncture points of Japanese quail (Coturnix coturnix japonica) egg quantity (egg production) and internal egg quality (Haugh unit, yolk index, and yolk colour). This research was conducted for 30 days using a sample of Japanese quail aged 4 weeks because for the first 2 weeks, the quail did not produce and it was in production in the last 2 weeks. There were 4 treatments and 25 replications each. So, there were a total of 100 quails. Laser puncture shooting was carried out at 3-day intervals at the Ova point and 6-day intervals at Hu Men, Bei Ji, and Wei Gen points. So, on the first day, laser puncture shooting was carried out at 4 points, on the 4th day, only at 1 point, and then, on the 7th day, it returned to 4 points. And so, it was carried out for 4 weeks. T0 (−) was considered as a negative control because quails are not given laser puncture shooting; T0 (+) was considered as a positive control because quails are treated with laser puncture shooting which is deactivated or a dose of 0 Joule; T1 was a group treated with laser puncture shooting at a dose of 0.2 Joule, and T2 was a group treated with laser puncture shooting at a dose of 0.5 Joule. Then, the research results were analyzed using Analysis of Variance and followed by Duncan’s Multiple Range Test. Laser puncture shooting at the reproductive acupuncture point of Japanese quail (Coturnix coturnix japonica) can increase the quantity of eggs (
p
<
0.05
). While the results of the egg internal quality in the form of the Haugh unit value and the yolk index increased (
p
<
0.05
), the value of the yolk colour did not change compared to the control (
p
>
0.05
). Laser puncture shooting at the reproductive acupuncture point of Japanese quail (Coturnix coturnix japonica) can increase egg quantity (egg production) and internal egg quality (Haugh unit and yolk index) with the best dose of 0.5 Joule.
This research to know the stimulation of reproduction point with a semiconductor laserpuncture to estrous synchronization of Bali Cattle was conducted in Loka Penelitian Sapi Potong Grati-Pasuruan, East Java. Estrous synchronization is a technique to get the sign of estrous and ovulation (estrous outside of estrous cycle) to be easier to detect estrous, synchronization of breeding, and shorter of calving interval. Laserpuncture was applied on 8 head bali cattles at 14 reproduction acupuncture points, 10 second for each point, 3 times frequency with interval 1 day (20-24 times). The variabel of this assessment were respons and signs of estrous, synchronized of estrous with Chi square test and T-test method. Result showed that there was significant correlation between semiconductor laserpuncture with estrous synchronization (p < 0,05). The result of assessment 8 (100%) head bali cattle was estrous. The respons of estrous appeared 1 head (12,5%) on 5th day (4th day post laserpuncture), 4 head (50%) on 6th day (5th day post laserpuncture) 1 head (12,5) on 8th day (7th day post laserpuncture), and 2 head (25%) on 10th day (9th day post laserpuncture). This result showed that semi conductor laserpuncture technology was effective to stimulate estrous synchronization and resulting ovulation, with the evidence that conception was occurred.
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