Aim:This study was conducted to examine the potential of mycotoxin binder in ceasing zearalenone (ZEN) effect on mice reproduction. ZEN mycotoxin can induce reactive oxygen species that may cause damage and cell death. ZEN is estrogenic so that it may affect the reproductive organs failure.Materials and Methods:Mycotoxin binder administration to female mice exposed to ZEN was aimed to count the number of primary follicles, secondary follicles, tertiary follicles, de Graaf’s follicles, and the corpus luteum (CL). Negative control group (C) was not exposed to ZEN and without the administration of mycotoxin binders, while positive control group (C+) was exposed to 0.1 mg/mouse/day ZEN and without the provision of mycotoxin binders. Treatment groups (T1, T2, T3) were exposed to 0.1 mg/mouse/day ZEN and mycotoxin binders 0.5; 1; 2 mg/BW/day.Results:ZEN and mycotoxin binders administration was conducted for 10 days. The number of primary follicles, secondary, tertiary, de Graaf’s follicles and CL in negative control (C−) was 14.2±1.36, 11.2±0.28, 6.5±0.53, 7.5±0.74, and 2.3±0.35. The number in positive control (C+) group was as follows 7.1±0.12, 3.7±1.17, 3.8±1.21, 1.5±0.62, and 2.3±0.34. Results in treatment 1 (T1) were as follows 6.2±0.16, 5.2±0.16, 3.6±0.16, 2.6±0.19, and 2.6±0.10; in treatment 2 (T2) 7.8±0.28, 5.8±0.53, 3.7±0.26, 2.7±0.26, and 2.5±0.10; and in treatment 3 (T3) 8.4±0.34, 8.4±0.34, 4.6±0.34, 4.5±1.01, and 3.4±0.23.Conclusion:The number of follicles and CL more in line with increasing doses of mycotoxin binders. Required more than 2 mg/mouse/day mycotoxin binders to inhibit the effects of ZEN so that its can maintain the number of primary follicle, secondary follicle, tertiary follicle, the de Graaf’s follicle, and the number of CL in the ovary of ZEN-exposed female mice (Mus musculus).

The purpose of this research was to know the relationship between age with calving inteval (CI), days open (DO), and service per conception (S/C) in Friesian Holstein dairy cattle (FH). The research was started on Desember 2017 to January 2018 in PT. Greenfields Indonesia partnerships, KecamatanWagir Kabupaten Malang. The materials of the search used were 100 heads of dairy cattle which had at least two times of parturition. The method used in this research was a survey and data collection. The data were obtained from the records of reproduction. Data analysis was multiple linear regression using SPSS software 21.0 version. The results showed that the values of CI (434,9±58,9 days, 449,4±66,2 days, and 431,8±59,2 days), DO (218,9±58,7 days, 218,9±58,7 days, dan 217,6±54,1 days), dan S/C (3,2±1,8 times, 4,3±1,9 times, 2,6±1,1 times) for the ages of 4, 5, 6. The relationship between age with CI and DO were not significant (P>0,05), but the relationship between age with S/C was significant (P<0,05). The value of S/C increased on dairy cattle at age of 4 to 5 years and started to decreased at the age of 6 years.

Reproduction is one of essential factor directly affecting profitability in a dairy production system. Some of the reproductive factors that influenced to the developing of dairy cattle of small holder’s farmer are Conception Rate (CR) Service per Conception (S/C). The study was conducted to know the CR and S/C of Friesian Holstein in KUD Argopuro, Probolinggo. The data is taken primary and secondary data. Primary data retrieval is done by direct observation, which includes several variables, where the variables include : the identity of the breeder, cages, and feeding and drinking. As for the secondary data obtained by recording the card Artificial Insemination (AI) owned by Inseminator in KUD Argopuro. The data which by collected from this sample are CR and S/C. This research was a case study. The materials used cattle as the acceptors were 10% from 728 lactation cattle. Data from the results of this research on CR (30%) and S/C (2,49). The conclusion of this research for CR the result were lower than normal and S/C the result were higher than normal.

This study examined effect of mycotoxin binder administered to female mice exposed to zearalenon on apoptosis incidence by observe at MDA, Caspase 9 and 3 levels in mice uterus. Negative control group (K−) was not exposed to zearalenon and without the administration of mycotoxin binders, positive control group (K+) exposed to zearalenon of 0.1 mg/head/day and without the provision of mycotoxin binders; and treatment groups (P1, P2, P3) were exposed to zearalenon 0.1 mg/head/day by providing mycotoxin binders each 0.5; 1; 2 mg/head/day. Zearalenon and mycotoxinbinders administration was conducted for 10 days. Results on MDA level were as follows 15.48 ± 0.50 (K−), 45.59 ± 0.50 (K+), 34.92 ± 3.38 (P1), 27.72 ± 1.25 and 23.89 ± 3.74. Caspase 9 levels showed the following results: 0.3 ± 0.60 (K), 8.3 ± 0.90 (K+), 3.6 ± 0.41 (P1), 3.3 ± 0.34 (P2) and 2.8 ± 0.28 (P3), while the levels of Caspase3 were as follows: 3.35 ± 0.44 (K−),12.5 ± 0.66 (K+), 3.6 ± 0.41 (P1), 4.80 ± 0.43 (P2) and 3.85 ± 0.50 (P3). In conclusion, mycotoxin binders may lower malondialdehyde (MDA) level and the expression of caspase 9 and caspase3 in the uterus of mice exposed to zearalenon.

This case report aims to determine the response of estrus and pregnancy rates in dairy cows with ovarian hypofunction by administering a combination of gonadotropin hormones. The sample used was ten dairy cows that had been calving, were not pregnant, showed no signs of estrus for more than 60 days after the last calving and had ovarian hypofunction based on rectal palpation. All dairy cows were injected 300 IU PG-600, then divided into two groups, each injected with 100 and 300 IU hCG respectively for first group and second group, intramuscularly at the time of insemination. The results showed that the estrus response occurred in all cows, in 6-8 (6.8 ± 0.84) days (first group), and 6-7 (6.2 ± 0.45) days (second group). Trans-rectal palpation pregnancy diagnosis 60 days after artificial insemination showed that all dairy cows in both groups were pregnant. It could be concluded that the treatment of dairy cows suffering from ovarian hypofunction with a combination of 300 IU PG-600 and 100 IU hCG was sufficient to induce 100% estrus rates and 100% pregnancy rates.

Demand for duck eggs and meat recently increased along with the increasing interest of the community to consume duck eggs and meat. Due to that reason researcher try to find the best way to enhance the reproductive ability of livestock., which is in this research by laser puncture shot. Eighteen ducks were allocated into three groups, the control group, the first treatment group with laserpuncture shoot dose of 0.2 Joule ( J), and the second treatment group with laser puncture shoot dose of 0.4 J. Data were analyzed with ANOVA and further tested by Duncan multiple range test. Research result showed that laser puncture shoot with a dose of 0.2 J and 0.4 J decreased the number of Sertoli and Leydig cells.

Semen storage in cold temperatures might cause an increase in reactive oxygen species (ROS) production. This condition resulted in spermatozoa damage and quality decrease. This study was conducted to investigate the effects of α-tocopherol supplementation in diluents on the motility, viability, and plasma membrane integrity of Simmental bull spermatozoa after cooling. Semen samples were diluted in skim milk egg yolk supplemented with 0, 0.5, 1.0, and 1.5 mM α-tocopherol respectively for control, Tl, T2, and T3. Spermatozoa were evaluated for their motility, viability, and membrane integrity in cooling temperature (5°C). The daily evaluation showed that 1.5 mM α-tocopherol was the best in maintaining motility, viability, and plasma membrane integrity, while 1.0 mM α-tocopherol was only good for maintaining viability. Therefore, it can be concluded that α-tocopherol at the concentration of 1.5 mM was an efficient antioxidant supplement for Simmental cattle semen in skim milk egg yolk diluent.

This study was conducted to determine the relationship between the estrous detector's reading value (Draminski®, Dramiński S.A, Poland) and serum progesterone levels on Etawa crossbred does. Eighteen healthynon-prehnant Etawa crossbreds does weighing 20-25 kg, in their first parity were used in this study. The does were divided equally into two groups. In the two groups, the does were synchronized their estrus by means of intramuscular and intravulvar injection of 7.5 mg prostaglandin Fɑ (PGF2ɑ) twice in 11-day intervals. After the second injection, the estrous detector value measurement, visual observation of the estrus sign, and blood sampling were conducted for five days. The blood sample was processed into the serum to examine for progesterone levels by ELISA. The data analysis was using the simple correlation method on SPSS type 23 for windows. The result showed that a positive relationship (p <0.05) with coefficient correlation (r) was 0.609 between the values of the estrous detector measurement with serum progesterone levels. It could be concluded that the estrous detector's low value indicates that the Etawa crossbred was in the estrus phase and followed by the decreasing of progesterone level but not reaching the basal level.