A aprendizagem ao longo de toda a vida é um novo paradigma e um princípio organizador para os sistemas de educação e aprendizagem frente ao século XXI proposto pela UNESCO. Já o letramento digital envolve outras novas competências e podem auxiliar no uso das Tecnologias da Informação e Comunicação para o benefício na vida pessoal e coletiva. Propõe-se realizar uma intervenção de alfabetização e letramento digital para idosos com duas metodologias educativas, com e sem utilização de aplicativo.
Plasma in several organisms has components that promote resistance to envenomation by inhibiting specific proteins from snake venoms, such as phospholipases A 2 (PLA 2 s). The major hypothesis for inhibitor's presence would be the protection against self-envenomation in venomous snakes, but the occurrence of inhibitors in non-venomous snakes and other animals has opened new perspectives for this molecule. Thus, this study showed for the first time the structural and functional characterization of the PLA 2 inhibitor from the Boa constrictor serum (BoaγPLI), a non-venomous snake that dwells extensively the Brazilian territory. Therefore, the inhibitor was isolated from B. constrictor serum, with 0.63% of recovery. SDS-PAGE showed a band at~25 kDa under reducing conditions and~20 kDa under non-reducing conditions. Chromatographic analyses showed the presence of oligomers formed by BoaγPLI. Primary structure of BoaγPLI suggested an estimated molecular mass of 22 kDa. When BoaγPLI was incubated with Asp-49 and Lys-49 PLA 2 there was no severe change in its dichroism spectrum, suggesting a non-covalent interaction. The enzymatic assay showed a dose-dependent inhibition, up to 48.2%, when BoaγPLI was incubated with Asp-49 PLA 2 , since Lys-49 PLA 2 has a lack of enzymatic activity. The edematogenic and myotoxic effects of PLA 2 s were also inhibited by BoaγPLI. In summary, the present work provides new insights into inhibitors from non-venomous snakes, which possess PLIs in their plasma, although the contact with venom is unlikely. OPEN ACCESS Citation: Rodrigues CFB, Serino-Silva C, Morais-Zani Kd, Kavazoi VK, Carvalho MPN, Grego KF, et al. (2020) BoaγPLI: Structural and functional characterization of the gamma phospholipase A2 plasma inhibitor from the non-venomous Brazilian snake Boa constrictor. PLoS ONE 15(2): e0229657. https://doi.org/10.
O objetivo é analisar os dados obtidos sobre curso de capacitação de recursos digitais para empreendedoras com idade acima de 50 anos, em termos de aprendizagem, ganhos pessoais e profissionais. Amostra formada por 28 empresárias, com idade igual ou superior a 50 anos, residentes na cidade de São Paulo. Como resultados e discussão, destaca-se que 80% das alunas afirmaram que iriam aplicar os conhecimentos obtidos no dia a dia de suas empresas, demonstrando a pertinência do conteúdo com a prática das empreendedoras.
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