Obesity is a condition involving low-level chronic inflammation as indicated by increased levels of C-reactive protein (CRP), tumor necrosis factor alpha (TNF-a), interleukin (IL) and other inflammatory markers in the blood. Some endocrine mediators, such as paracrine and autocrine play an important role in regulating the function of adipocytes, especially related to insulin sensitivity. The purpose of this study is to determine the level CRP, TNF-a, IL-6, resistin and insulin resistance in the obese Javanese population. This was a preliminary study involving 120 people, consisting of 60 obese subjects and 60 non obese subjects as controls. Lipid profiles, CRP, TNF-a, IL-6, resistin levels were determined with ELISA methods, whereas insulin resistance was calculated by HOMA IR index. The study found that the glucose, insulin, CRP levels and HOMA-IR of obese subjects were significantly higher than those non obese subjects (p<0.05). However, the TNF-a, IL-6 and resistin levels were not significantly different between obese and non obese subjects (p>0.05).In conclusion, the CRP levels ad insulin resistance in obese Javanese population were higher compared with those non obese. ABSTRAKObesitas merupakan suatu kondisi yang melibatkan peradangan kronis tingkat rendah seperti ditunjukkan oleh peningkatan kadar protein C-reaktif (CRP), tumor necrosis factor alpha (TNF-a), interleukin (IL) dan penanda inflamasi lainnya dalam darah. Beberapa mediator endokrin, seperti parakrin dan autokrin berperan penting dalam mengatur fungsi adiposit, terutama yang berkaitan dengan sensitivitas insulin. Tujuan penelitian ini adalah untuk menetapkan kadar CRP, TNF-a, IL-6, resistin dan resistensi insulin pada populasi Jawa obes. Penelitian ini merupakan studi pendahuluan yang melipatkan 120 subjek, terdiri dari 60 subjek obes 60 tidak obes sebagai kontrol. Profil lipid, kadar CRP, TNF-a, IL-6, dan resistin diukur dengan metode ELISA, sedangkan resistensi insulin dihitung dengan indeks IR HOMA. Dari penelitian ini ditemukan bahwa kadar glukosa, insulin, CRP, dan HOMA-IR subjek obes lebih tinggi secara bermakna dibandingkan subjek non obes (p<0,05). Namun demikian kadar TNF-a, IL-6 dan resistin tidak berbeda bermakna antara kelompok subjek obes dan non obes (p> 0,05). Dapat disimpulkan, kadar CRP dan resistensi insulin pada populasi Jawa obes lebi tinggi dibandingkan dengan mereka yang tidak obes.

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Tumor necrosis factor-α (TNF-α) is an adipokine produced in adipocytes which

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One of the main causes of women's cancer-related death is Epithelial Ovarian Cancer (EOC).A new spectrophotometric method was developed to determine a biomarker for EOC. This study aims to investigate the pattern of EDTA-blood photo spectrum in EOC patients. One ml blood was collected from sixty-six subjects (33 EOC patients, 33 healthy controls) by venipuncture, which then drawn into EDTA tubes for photo spectrum analysis. The t-test using programming in Matrix Laboratory (MATLAB) for Windows 7 tested the differences in ENBS and EBS parameters in the two groups and the parameters of EDTA-blood photo spectrum in two groups based on the energetics of non-biological systems (ENBS) and energetics of biological systems (EBS) approach. The statistical significance of p<0.01 was taken to evaluate the difference. The receivers operating characteristic (ROC) curves were applied to evaluate the effect of biomarkers on these parameters. The area under the ROC curve (AUC) is used to evaluate the biomarker with the correspondence interval (CI) of 95%. The t-test results indicate that the significant difference was observed between EOC patients and healthy controls only in parameter-1 of the parameters based on the ENBS approach. However, there were significant differences in all parameters based on the EBS approach. In a training dataset, the AUC values were 0.663, 0.704, 0.546, 0.611, 0.619, and 0.676 for ideal parameter-1 to 6 (IP1-6); and 0.886, 0.855, 0.765, 0.909, 0.897, and 0.789 for real parameter-1 to 6 (RP1-6). Sensitivity and specificity of IP1-