When grown in alternating cycles of light and darkness, non-synchronous cultures of Gloeotkce fixed N2 mainly in the dark phase. This diumd pattern of N2 fixation was independent of the doubling time (69 & 16 h) of the organism and, since cell divisionwas asynchronous, N2 fixation does not seem to be confined to a specific phase in the cell cycle. Fluchrtltions in the rate of N2 fixation coincided with similar fluctuations in the rate of nitrogenase synthesis. Diurnal fluctuations also occurred in the utilization of &can and acid-soluble polyphosphate and in the ADP/ATP ratio. Based on these observations, it is pmposed that specific mcfabofic changes are involved in the regulation of the diurnal pattern of N2 fixation by Glcnmthece.

No abstract

Bacillus sp has been isolated locally from Sulaimani soil sample using nutrient agar plates. The isolate was identified as Bacillus genus according to the morphological feature of the colonies and cells. The bacterial growth was obtained using nutrient broth at 37 ºC of pH 7.2. Adenosine 3',5'-cyclic monophosphate was extracted from the broth medium using incubation periods of 6, 12, 18 and 24 h. The extract was purified using Dowex-50 H from column chromatography technique. The purified adenosine 3',5'-cyclic monophosphate was identified using UV, HPLC and TLC analysis.