Adenosine 3',5'-Cyclic Monophosphate Extracted from Local Isolate of Bacillus Species

Abstract

Bacillus sp has been isolated locally from Sulaimani soil sample using nutrient agar plates. The isolate was identified as Bacillus genus according to the morphological feature of the colonies and cells. The bacterial growth was obtained using nutrient broth at 37 ºC of pH 7.2. Adenosine 3',5'-cyclic monophosphate was extracted from the broth medium using incubation periods of 6, 12, 18 and 24 h. The extract was purified using Dowex-50 H from column chromatography technique. The purified adenosine 3',5'-cyclic monophosphate was identified using UV, HPLC and TLC analysis.