Cell refractive index is a key biophysical parameter, which has been extensively studied. It is correlated with other cell biophysical properties including mechanical, electrical and optical properties, and not only represents the intracellular mass and concentration of a cell, but also provides important insight for various biological models. Measurement techniques developed earlier only measure the effective refractive index of a cell or a cell suspension, providing only limited information on cell refractive index and hence hindering its in-depth analysis and correlation. Recently, the emergence of microfluidic, photonic and imaging technologies has enabled the manipulation of a single cell and the 3D refractive index of a single cell down to sub-micron resolution, providing powerful tools to study cells based on refractive index. In this review, we provide an overview of cell refractive index models and measurement techniques including microfluidic chip-based techniques for the last 50 years, present the applications and significance of cell refractive index in cell biology, hematology, and pathology, and discuss future research trends in the field, including 3D imaging methods, integration with microfluidics and potential applications in new and breakthrough research areas.

Metamaterials have attracted intensive research interest in recent years because their optical properties have a strong dependence on the geometry of metamaterial molecules rather than the material composition. [1][2][3] This feature has inspired the creation and tailoring of exotic properties, such as a negative refractive index, [ 4 , 5 ] perfect absorption, [ 6 ] and super lensing, [ 7 , 8 ] which are not readily available in nature. For many practical applications such as data storage [ 9 ] and optical switching, [ 10 ] switchable metamaterials that possess very different states are almost a necessity. [ 11 ] Most of the tunable metamaterials that have been demonstrated rely on tuning constituent materials or changing surrounding media by introducing natural materials with higher tunability, such as liquid crystals and phase changing materials. [12][13][14][15][16][17][18][19] However, this limits the choices of materials and becomes increasingly diffi cult to implement at higher frequencies. Moreover, the tuning range is usually too limited to achieve a switching effect between strikingly different states.A complementary approach is to mechanically reconfi gure the metamaterial molecules. [ 20 , 21 ] Micromachining technology has been developed for fabrication and actuation of micromechanical devices [22][23][24][25][26] with switching frequencies up to the GHz level. [ 27 ] An attempt was made to adjust the distance between several planar metamaterial layers in which effi cient transmission change was achieved but the tuning originated from a change in the layer structure rather than a change in metamaterial molecule. [ 22 ] Recently, another interesting work demonstrated the modifi cation of the optical properties of a metamaterial by reorienting the metamaterial molecules. [ 23 ] Inspired by these prior studies, we report the concept and design of switchable magnetic metamaterials by directly reshaping the metamaterial molecules using the micromachining technology and present working devices with switchable magnetic responses.The schematic diagram of the switchable magnetic metamaterial is shown in Figure 1 a. Each metamaterial molecule consists of two semi-square split rings. One is anchored on the substrate while the other can be moved by micromachined actuators. As a result, the gap between the split rings can be altered and thus the geometric shape of the metamaterial molecule can be changed. Figure 1 b-d illustrates the two semi-square spit rings in different states. In Figure 1 b, the two split rings are separated by a small gap, resulting in a geometric shape "[]". This is a typical split ring resonator. [ 28 ] For simple notation, this state is called the open-ring state. Figure 1 c,d show two extreme cases. In the former, the gap between the two split rings is closed and the actual metamaterial molecule becomes a closed ring in the "ٗ" shape. This is called the closed-ring state. In the latter, the movable ring is moved away until it touches the back side of the fi xed ring in the next metama...

Dichroic polarizers and waveplates exploiting anisotropic materials have vast applications in displays and numerous optical components, such as filters, beamsplitters and isolators. Artificial anisotropic media were recently suggested for the realization of negative refraction, cloaking, hyperlenses, and controlling luminescence. However, extending these applications into the terahertz domain is hampered by a lack of natural anisotropic media, while artificial metamaterials offer a strong engineered anisotropic response. Here we demonstrate a terahertz metamaterial with anisotropy tunable from positive to negative values. It is based on the Maltese-cross pattern, where anisotropy is induced by breaking the four-fold symmetry of the cross by displacing one of its beams. The symmetry breaking permits the excitation of a Fano mode active for one of the polarization eigenstates controlled by actuators using microelectromechanical systems. The metamaterial offers new opportunities for the development of terahertz variable waveplates, tunable filters and polarimetry.

This paper presents a novel silicon-based and batch-processed MEMS electrostatic transducer for harvesting and converting the energy of vibrations into electrical energy without using an electret layer. Effective conversion from the mechanical-to-electric domains of 61 nW on a 60 M resistive load, under a vibration level of 0.25 g at 250 Hz, has been demonstrated. Rigorous analysis of the efficiency of the harvester is presented, covering issues related with mechanical and electrical operation. Various schemes for the conditioning electronics are discussed and the harvested power measurements using a dc/dc converter are explained in detail. The paper concludes with a comparison with previous electrostatic transducers based on a new simple factor of merit.

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Metastasis is the main cause of cancer mortality. During this process, cancer cells dislodge from a primary tumor, enter the circulation and form secondary tumors in distal organs. It is poorly understood how these cells manage to cross the tight syncytium of endothelial cells that lines the capillaries. Such capillary transmigration would require a drastic change in cell shape. We have therefore developed a microfluidic platform to study the transmigration of cancer cells. The device consists of an array of microchannels mimicking the confined spaces encountered. A thin glass coverslip bottom allows high resolution imaging of cell dynamics. We show that nuclear deformation is a critical and rate-limiting step for transmigration of highly metastatic human breast cancer cells. Transmigration was significantly reduced following the treatment with a protein methyltransferase inhibitor, suggesting that chromatin condensation might play an important role. Since transmigration is critical for cancer metastasis, this new platform may be useful for developing improved cancer therapies.

This paper presents an advanced study including the design, characterization and theoretical analysis of a capacitive vibration energy harvester. Although based on a resonant electromechanical device, it is intended for operation in a wide frequency band due to the combination of stop-end effects and a strong biasing electrical field. The electrostatic transducer has an interdigited comb geometry with in-plane motion, and is obtained through a simple batch process using two masks. A continuous conditioning circuit is used for the characterization of the transducer. A nonlinear model of the coupled system ‘transduce-conditioning circuit’ is presented and analyzed employing two different semi-analytical techniques together with precise numerical modelling. Experimental results are in good agreement with results obtained from numerical modelling. With the 1 g amplitude of harmonic external acceleration at atmospheric pressure, the system transducer-conditioning circuit has a half-power bandwidth of more than 30% and converts more than 2 µW of the power of input mechanical vibrations over the range of 140 and 160 Hz. The harvester has also been characterized under stochastic noise-like input vibrations.

The aim of this paper is to present a rapid modeling method for micropumps, based on electrical analogies and equivalent networks. The different parts of a pump are modelled by means of lumped elements which are calculated using appropriate formulae. These elements are linked together to constitute a network which describes the entire electromechanical system. The networks built were implemented in an electrical simulation tool for rapid analysis. In the case of pneumatic actuation, the method was validated by a qualitative and quantitative comparison with results obtained by another method. The particular case of electrostatic actuation was also investigated, leading to the observation of specific features of this actuation mechanism.