In order to study productive performance and meat quality of slow-growing chicks used for rural poultry production in relation to methionine and enzyme supplementations, basal diets were formulated to contain 0.32, 0.29 and 0.27% methionine levels from 1 to 42 d of age, 43 to 84 d of age, and 85 to 98 d of age, respectively. These diets were supplemented with 0, 0.06 and 0.12% of DL-methionine; thus, there were three methionine levels during each period. These levels were fed with or without 750 FTY of phytase-6 or one g/kg of multienzymes; thus there were three methionine levels by three enzyme treatments in factorial design. Methionine levels of 0.44, 0.41 and 0.33% significantly increased productive performance from 1 to 42, 43 to 84 and 85 to 98 d of age, respectively. Phytase supplemented-basal diet and diet supplemented with 0.06% methionine and phytase yield similar productive performance to those of chicks fed diet supplemented with 0.06% and 0.12% methionine, respectively. Phytase improved significantly nutrient digestibilities, percentage dressed carcass and chest parts compared to the control and multienzymes groups. Increasing methionine levels by 0.06 and 0.12% improved significantly percentage dressed carcass, chest and hind parts by 2.2 and 2.7, 1.4% and 2.8 and 3.1 and 2.5%, respectively. However, the methionine level and/or type of enzyme supplementations did not significantly affect metabolic profiles such as plasma total protein, albumen, globulin, total lipids and cholesterol.
Thirty-six V-line male rabbits ten months age, average weight 3.5 kg were used. They were allocated randomly into six equal groups. First and second were served as a negative (NC) and positive (PC) control. Third group was injected by double dose (1mg/kg body weight) of ivermectin (IV) via subcutaneous injection, fourth, fifth and sixth groups were treated with 5% garlic oil (GO), 5% cinnamon oil (CO) and 5% garlic oil + 5% cinnamon oil mixture (MO), respectively. Rabbits were treated GO, CO and MO received daily topically treatment for 7 successive days. Groups PC and IV were treated with paraffin oil and ivermectin. Blood samples were collected at 1, 7 and 14 days of treatment to biochemical analysis. Semen characteristics and reaction time (libido) were recorded.Results showed that there were a significant (P≤0.05) decreased in total protein, albumin, globulin, testosterone, semen characteristics, TAC in rabbits infested groups compared with NC group. But there were a significant (P≤0.05) increase in ALT, AST, creatinine, urea and uric acid, cortisol, T 3 and T 4 , MAD in the rabbits infested groups compared with NC group. Rabbits treated with ivermectin recorded a significant (P≤0.05) decrease at 1 and 7 days in serum total protein, albumin compared with other groups, while, globulin decreased gradually from day 1, 7 and 14 comparable with other groups. Serum total protein, albumin, ALT, AST, creatinine, urea and uric acid were increased toward to NC group at 7 th and 14 th days of therapeutic.Garlic and cinnamon oils caused a decrease in ALT, AST, creatinine, urea and uric acid with time compared with PC and IV groups. While, injected ivermectin for infested rabbits led to significant (P≤0.
The present study aimed to compare the effects of bee venom (BV) and Oxytetracycline (OXY) supplementation on blood biochemical analysis, antioxidant, immunity status and bacterial count of weaning rabbits. Sixty Californian male rabbits at 35 days of age with average body weight of 589±90 g were randomly divided into five equal groups (12 for each); 1 st group (control) was given water (placebo), 2 nd group (OXY; 1g/l water), 3 rd , 4 th , and 5 th groups were received BV at (2, 4 and 8 mg/kg body weigh/day, respectively). Results indicated that weaning rabbits treated with OXY and BV had significant increases of total plasma protein (TP) and globulin (Glo) while decreasing AST and ALT, except for OXY group rise of ALT compared to control group. Rabbits treated with OXY or BV had a significantly declined tri-glycerids (TG), total cholesterol (TC) and very low-density lipoprotein (VLDLc), while all treatment records were insignificant for high-density lipoprotein (HDL-c) and low-density lipoprotein (LDL-c) compared to control group. Groups treated with BV showed increase of IgG, total antioxidant capacity (TAC), superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPX). Group treated with 2 mg BV/kg body weight/day had a decreased total bacterial count (TBC), salmonellae, E. coli, Proteus and Clostridia. Conclusively, it could be recommended the use levels of BV (2 mg BV/kg body weight/day) to improve the biochemical, immunological and antioxidative responses and decrease pathogenic bacteria in hindgut of weaning rabbits.
Twenty V-line male rabbits, ten months age and 80 multiparous does aged 12 months were used for the present study. Three experiments were carried out. First experiment was planned to use honey bee as a component in sperm preservation extender at incubation (37°C) for up to 6 hours or refrigeration condition (4-6°C) for up to 72 hours. Second experiment was done to study effect of different levels of honey bee on total bacterial count, enterobacteriaceas count, Staph. aureus. Third experiment was designed for studying fertility traits for does artificially inseminated by diluted pooled semen with antibiotics (control) and the best level of honey bees.Results showed that diluted V-line rabbits semen with 1, 3, or 5 ml honey bee/100ml extender improved significantly (P≤0.05) semen quality increased in percentages of advanced sperm motility and decreased percentages of dead spermatozoa, sperm abnormalities and acrosomal damages during cryopreservation at (4-6°C) for up to 72 hours or incubation at 37°C for up to 6 hours.Addition of different levels of honey bee to rabbits semen extender resulted in a gradually and significantly decrease (P≤0.05) in overall mean of MAD concentration. Total anti-oxidant enzymes activity of rabbit semen extender was increased by increasing honey bee level and this effect was significant (P≤0.05), regardless the incubation or storage time. Moreover, the results revealed that the highest honey bee levels had the highest total anti-oxidant capacity value, which was significant (P≤0.05) compared to the other levels. Honey bee at different concentrations, generally, had a broad action against total bacterial count of V-line rabbit semen, during chilled storage at 4-6°C or incubation at 37°C. Manuka honey and its antimicrobial potential against multi drug resistant strains of Typhoidal salmonellae, Ph.D. Thesis, Department of Microbiology, University of Health Science, Lahore, Pakistan. Hassan, M.I.; G.M. Mabrouk; H.H. Shehata and M.M. Aboelhussein (2010). Antineoplastic effects of bee honey and Nigella sativa on hepatocellular carcinoma cells. Integr. Cancer Ther., 10.1177/ 1534735410387422 . Januskauskas, A. and H. Zillinskas (2002). Bull semen evaluation post-thaw and relation of semen characteristics to bull's fertility. Veterinari Jair Zootechnika, 17: 1392-2130. Kacániová, M.; N. Vuković; R. Chlebo; P. Haščík; K. Rovná; J. Cubon; M. Dżugan and A. Pasternakiewicz (2012). The antimicrobial activity of honey, bee pollen loads and bees wax from Slovakia. Arch. Biol. Sci., Belgrade, 64: 927-934. Kankofer, M.; G. Kolm; J. Auric h and C. Auric h (2005). Activity of glutathione peroxidase, superoxide dismutase and catalase and lipid intensity in stallion semen during storage at 5°C. Theriogenology, 4: 63-135. Kaur, M.; K.K. Tripathi; M.R. Bansal; P.K. Jain and K.G. Gupta (1986). Bacteriology of cervix incases of infertility: effect on human sperm. Am. A.S. Halim; M.S. Syaz ana and K.N. Sirajudeen (2011). Tualang honey has higher phenolic content and greater radical scavenging activity compared ...
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