Ensiling characteristics, structural and nonstructural carbohydrate composition and enzymatic digestibility (ED) of Napier grass silage was examined. Napier grass ensiled with no additive control, 0.2% formic acid, 0.4% molasses, and 0.3% fibrolytic enzyme for, 7, 30, 60 and 90days. Additives increased lactic acid, soluble carbohydrate and decreased all of lignocellulosic contents except acid detergent lignin and pH than control. The highest value of nonstructural carbohydrate and large reduction in lignocellulosic contents was observed in formic acid and fibrolytic enzyme silage respectively. The content of glucose and fructose showed rapid drop in the first 7days of ensilage. Ensilage decreased lignocellulosic contents and increased ED compared to fresh material. The ED of formic acid and molasses silage was significantly higher than control and fibrolytic enzyme silages in all tested days. In summery the ensiling quality structural and nonstructural carbohydrate and ED value of mature Napier grass silage improved through additives.

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Our findings show that TMR prepared with oat-common vetch intercrop can be well preserved. Although propionic acid is compatible with lactic acid bacteria, and when used together, they had minor effects on fermentation, aerobic stability and in vitro digestibility of TMR silage prepared with oat-common vetch intercrop.

In order to evaluate the nutritional value and digestibility of corn (Zea mays L.) stover silage mixed with legume herbages in Tibet, corn stover was ensiled with four levels (0, 10, 20 and 30% of fresh weight) of common vetch (Vicia sativa L.) and alfalfa (Medicago sativa L.), respectively. The silos were opened after 45 days of ensiling, and the fermentation characteristics, nutritive quality and in vitro digestibility of the mixed silages were analyzed. The results showed that the inclusion of legumes could somewhat improve the corn stover silage quality, as indicated by significantly (P < 0.05) higher lactic acid and crude protein contents and ratios of lactic acid/acetic acid, and significantly (P < 0.05) lower amylase‐treated neutral detergent fiber, acid detergent fiber and hemicellulose contents, while the corn stover silage containing 10% common vetch performed best, as indicated by significantly (P < 0.05) higher in vitro dry matter and neutral detergent fiber digestibility. These results suggest that mixing legumes to corn stover before ensiling appears to be a feasible strategy to improve the nutritive quality of mixed silage.

Freezing damages in forages represents a major economic loss to agriculture. This study was conducted to investigate the effects of freeze–thaw (FT) event on microbial community dynamics of red clover silage. Results showed that the FT-treated material displayed higher proportions of Weissella and aerobic bacteria, while lower Pantoea and Enterobacter compared with the control material. The FT event promoted the development of Lactobacillus in silage microflora, inducing more intense lactic fermentation after an initial short lag. The aerobic bacteria were suppressed immediately after the onset of ensiling. Microbiomes of the two silages tended to be almost similar after 2 days of ensiling. However, a small number of aerobic bacteria tended to revitalize in the FT silage with prolonged ensiling time, indicated by apparent abundances of Acinetobacter and Pseudomonas at the end of ensiling. The results obtained here suggest that the FT event could promote the development of Lactobacillus during ensiling and the control of aerobe revitalization need to be concerned with silages made from the freeze-damaged forages.

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BACKGROUND: Silages produced with grass and legume mixtures may have positive associative effects on silage quality and animal performance when ruminants are fed the silages. The present study aimed to determine the preservation characteristics, in vitro rumen fermentation profile and microbial abundance of silages, produced with mixtures of sweet sorghum (SS) and alfalfa (AF) in different ratios: 100:0 (Control), 75:25 (SA25), 50:50 (SA50), 25:75 (SA75) and 0:100 (SA100) on a fresh weight basis. RESULTS: As the proportion of AF increased in the silages, pH, acetic acid, ammonia nitrogen and crude protein (CP) concentrations, and aerobic stability increased (P < 0.05), whereas lactic acid and neutral detergent fibre (NDF) and acid detergent fibre (ADF) concentrations decreased (P < 0.05). Increasing the proportion of AF in the silages, increased (P < 0.05) the in vitro degradability of dry matter and CP, as well as the proportions of genus Prevotella, but decreased (P < 0.05) the in vitro degradability of NDF (IVNDFD) and ADF (IVADFD) and proportions of Butyrivibrio fibrisolvens. Compared to the Control silage, SA25 silage had greater (P < 0.05) proportions of Fibrobacter succinogenes and Ruminococcus flavefaciens, IVNDFD, IVADFD, ruminal short chain fatty acids and microbial protein concentrations, as well as lower (P < 0.05) methane production. CONCLUSION: The silage produced with the SS to AF ratio of 75:25 was the most suitable for ruminants use as a result of the optimal balance of fermentation quality, feed-nutritional value and aerobic stability.

Twelve Holstein bulls were used in a 4 × 4 Latin square design to investigate the effects of using whole-crop wheat hay (WCWH) as a substitute for Leymus chinensis (LC) on apparent digestibility, plasma parameters, ruminal fermentation, and microbial communities. Experimental treatments were four proportions of WCWH, 0, 33, 67, and 100%, as a substitute for LC (WCWH0, WCWH33, WCWH67, and WCWH100, respectively). The WCWH100 group showed a higher nutritional intake of crude protein (CP) and higher apparent digestibility of organic matter (OM), CP, and ether extract (EE) than the WCWH0 group (P < 0.05). Urea N, NH3-N, isobutyrate and isovalerate levels were higher (P < 0.05) in the WCWH100 group than in the WCWH0 group. 16S rRNA high-throughput sequencing analysis revealed similarities in the community composition, species diversity and relative abundance of dominant bacteria at the phylum and genus levels among the four groups. Collectively, our data indicated that WCWH can be used to replace LC in the diet of finishing dairy bulls without having a negative impact on apparent digestibility, plasma parameters, and ruminal bacteria composition. These results offer the first deep insight into the effects of replacing LC with WCWH on the performance parameters and rumen microbiota in Holstein bulls, and may aid in ruminant farming.