e15033 Background: Cardioesophageal cancer is a common tumor affecting the cardiac stomach and lower esophagus. Tumor heterogeneity is important, since it can cause the ineffectiveness of chemotherapy and radiotherapy due to individual characteristics of the neoplasm in different patients, as well as the intensification of invasion and metastasis. Our purpose was to analyze the relative copy numbers of TP63, YAP1 and KMT2D genetic loci and EGFR expression in orthotopic patient-derived xenografts of cardioesophageal cancer. Methods: The model of cardioesophageal cancer was created in Balb/c Nude mice with surgical bioptates of adenocarcinoma obtained from donors. The relative copy numbers of TP63, YAP1 and KMT2D genetic loci and EGFR expression were determined by Real-Time qPCR. Results: A PDX model of cardioesophageal cancer was successfully created by transplanting a moderately differentiated adenocarcinoma from a patient diagnosed with infiltrative ulcerative cancer of the lower third of the esophagus with a transition to the cardiac stomach into the distal esophagus of Balb/c Nude mice. The EGFR expression was elevated in patient tumor samples, compared to healthy tissues (p = 0.021) and gastric cancer xenografts (p = 0.07). Molecular genetic analysis demonstrated an association between an increased EGFR expression and changes in the relative copy numbers of TP63, YAP1, and KMT2D in donor samples compared to gastric cancer xenografts (p = 0.02, p = 0.026 and p = 0.042). Conclusions: The relative copy numbers of TP63, YAP1, and KMT2D were associated with intensified EGFR expression and changed with each new generation of orthotopic xenografts.
e15034 Background: Cardioesophageal cancer is one of the most common tumors affecting the mucosa of the cardiac stomach and distal esophagus. Despite the variety of treatment strategies and chemotherapy agents, the prognosis for the patients remains poor. The purpose of the study was to analyze the relative copy numbers of SOX-2 and NOTCH1 and vimentin expression in orthotopic patient-derived xenografts of cardioesophageal cancer. Methods: The model of cardioesophageal cancer was created in Balb/c Nude mice with surgical bioptates of moderately differentiated adenocarcinoma obtained from a donor with infiltrative ulcerative cancer of the lower third of the esophagus with a transition to the cardiac stomach into the distal esophagus of Balb/c Nude mice. The index of proliferative activity in the bioptates was assessed by IHC. The relative copy numbers of SOX-2 and NOTCH1 and vimentin expression were determined by Real-Time qPCR. Results: Expression of vimentin was absent in tissues of the donor tumor. The levels of vimentin expression statistically significantly increased in xenografts (1+ and 3+). The SOX-2 and NOTCH1 relative copy numbers were statistically significantly increased in tissues of the donor tumor (0.9 and 0.7), compared to xenografts (1.5±0.03 and 1.7±0.03). Molecular genetic analysis demonstrated an association between an increased vimentin expression and changes in the relative copy numbers of SOX-2 and NOTCH1 (p = 0.013 and p = 0.0001). Conclusions: The relative copy numbers of SOX-2 and NOTCH1 genetic loci was associated with increased expression of the epithelial-mesenchymal transition marker vimentin in tumor tissue samples and changed with each new generation of orthotopic xenografts.
Несмотря на то что с каждым годом растет количество сообщений об эффективности и безопасности ССДР ПЖ, еще не представляется возможным однозначно судить о том, оправданно ли выполнение ССДР ПЖ при НЭО. В данной статье приводятся результаты ретроспективного исследования, проведенного на базе ФГБУ «НМИЦ онкологии» МЗ РФ в период с 2011 по 2020 год, в которое было включено 9 пациентов, прооперированных методом ССДР ПЖ. Среднее время оперативного вмешательства составило 155 минут в группе открытых ССДР ПЖ и 147 минут в группе лапароскопических ССДР ПЖ. Уровни кровопотери составили 110 мл и 115 мл при открытых и лапароскопических ССДР ПЖ соответственно. Среднее время пребывания пациентов в стационаре после открытой ССДР ПЖ составило 10 дней, тогда как у пациентов после лапароскопической ССДР ПЖ -7 дней. На сегодняшний день среднее время наблюдения составляет 85 месяцев, показатель 5летней выживаемости в группе пациентов после открытой ССДР ПЖ достигает 96,5%, а в группе после лапароскопической ССДР ПЖ -100%. Все пациенты чувствуют себя удовлетворительно, регулярно проходят контрольные обследования и наблюдаются у онколога по месту жительства. Ключевые слова: нейроэндокринные опухоли, сплен-сохраняющие резекции, 5-летняя выживаемость, лимфодиссекция.
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