ABSTRACT. Diversity of Pentatomoidea (Hemiptera, Heteroptera) in three fragments of Atlantic Forest in southern Santa Catarina, Brazil. The composition and the seasonal variation of Pentatomoidea (Hemiptera) were evaluated between September
RESUMOEstudou-se o cultivo da cianobactéria Spirulina platensis utilizando-se efluente sintético de suíno a fim de avaliar o crescimento das cepas LEB 52 e PARACAS dessa cianobactéria, em função da diluição do meio (0 e 50%) e da concentração de bicarbonato de sódio (0 e 3g/L). Nesta etapa foi utilizado um Planejamento Fatorial Completo 2 3 . Na Segunda etapa, diluiu-se o meio em 80, 50 e 20% e utilizou-se um Planejamento Fatorial Multiníveis 3 1 x 2 1 . Os cultivos foram realizados a 30°C, com fotoperíodo de 12 horas. As variáveis utilizadas como resposta nas duas etapas foram a concentração máxima de biomassa e a velocidade específica máxima de crescimento, sendo os resultados avaliados mediante de Análise de Variância. Na primeira etapa, os maiores valores de concentração celular, 0,55 g/L, foram obtidos com a cepa LEB-52 em efluente sintético diluído a 50% em água destilada e sem a adição de bicarbonato de sódio, como suplementação da fonte de carbono. Na otimização da diluição, o maior valor de concentração celular foi de 0,35 g/L utilizando-se cepa PARACAS e diluição de 80%. As duas cepas estudadas de Spirulina platensis podem crescer em efluente sintético de suíno e o seu crescimento é favorecido pela diluição do meio.Termos para indexação: Spirulina platensis, cianobactéria, efluente suíno. ABSTRACTThe growth of LEB-52 and PARACAS strains of Spirulina platensis cyanobacteria was studied using synthetic swine wastewater. The effects of medium dilution (0 and 50%) and sodium bicarbonate addition to the medium (0 and 3g/L) were initially investigated. In this step, it was used a complete Factorial Planning 2 3 . In the second step, the medium was diluted at 80, 50 and 20% and it was used a Factorial Planning 3 1 x2 1 . The cultivation was carried out at 30°C, with photophase of 12 hours. The maximum biomass concentration and maximum specific growth rate were assessed, with the results being evaluated through Analysis of Variance. In first step, the greater biomass concentration (0.55g/L) was obtained with strain LEB-52, in synthetic swine wastewater diluted at 50% and without the sodium bicarbonate addition, as supplementation of the carbon source. In the second step, the greater biomass concentration was 0.35g/L using strain PARACAS and synthetic swine wastewater diluted at 80%. The two strains studied can grow in the synthetic swine wastewater and cyanobacteria growth is enhanced by medium dilution.
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