Objectives: Indium compounds are used in manufacturing displays of mobile phones and televisions. However, these materials cause interstitial pneumonia in exposed workers. Animal experiments demonstrated that indium compounds caused lung cancer. Chronic inflammation is considered to play a role in lung carcinogenesis and fibrosis induced by particulate matters. 8-Nitroguanine (8-nitroG) is a mutagenic DNA lesion formed during inflammation and may participate in carcinogenesis. To clarify the mechanism of carcinogenesis, we examined 8-nitroG formation in indium-exposed cultured cells. Methods: We treated RAW 264.7 mouse macrophages with indium oxide (In2O3) nanoparticles (primary diameter: 30-50 nm), and performed fluorescent immunocytochemistry to detect 8-nitroG. The extent of 8-nitroG formation was evaluated by quantitative image analysis. We measured the amount of nitric oxide (NO) in the culture supernatant of In2O3-treated cells by the Griess method. We also examined the effects of inhibitors of inducible NO synthase (iNOS) and endocytosis on In2O3-induced 8-nitroG formation. Results: In2O3 significantly increased the intensity of 8-nitroG formation in RAW 264.7 cells in a dose-dependent manner. In2O3-induced 8-nitroG formation was observed at 2 h and further increased at 4 h, and the amount of NO released from In2O3-exposed cells was significantly increased at 2-4 h compared with the control. 8-NitroG formation was suppressed by 1400W (an iNOS inhibitor), methyl-β-cyclodextrin and monodansylcadaverine (inhibitors of caveolae- and clathrin-mediated endocytosis, respectively). Conclusions: These results suggest that endocytosis and NO generation participate in indium-induced 8-nitroG formation. NO released from indium-exposed inflammatory cells may induce DNA damage in adjacent lung epithelial cells and contribute to carcinogenesis.
A comparative technical and economic analysis of two communitybased seasonal floodplains (Khirai and Angrail) of Daudkandi Upazila, Comilla during 2008 to 2010, are represented in this paper. The two floodplains differed mainly in management and fish production which were related to capital investment, variable cost, share of profits and proportional reimbursement to the fishers. Local management committees have been developed in each floodplain with significant differences. Average 142 kg/ha of fish fingerlings (carps such as Catla catla, Labeo rohita, Cirrhinus cirrhosus, Labeo calbasu, Labeo bata and exotic species such as Hypopthalmicthys molitrix, Aristichthys nobilis, Cyprinus carpio, Ctenopharyngodon idella, Pungasius hypophthalmus, Barbonymus gonionotus, Oreochromis niloticus) were stocked at the varying proportions. Fish production obtained were 2.8 to 4.4 mt/ha and 1.1 to 1.3 mt/ha in Khirai and Angrail floodplain, respectively. The average cost of fish production, total income and the net income have been estimated as Tk. 209650 ± 54167.12/ha, Tk. 281306 ± 88263.42/ha and Tk. 71656 ± 35152.51/ha in Khirai floodplain and Tk. 62349 ± 5768.90/ha, Tk. 111930 ± 12711.02/ha and Tk. 49580 ± 6948.26/ha in Angrail floodplain, respectively. The benefit cost ratio (BCR) has been recorded as 1.33 and 1.79 in Khirai and Angrail floodplain, respectively. The results reveal that the fish production and economic benefit was higher in Khirai floodplain.
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