As of March 24, 2020, novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been responsible for 379,661 infection cases with 16,428 deaths globally, and the number is still increasing rapidly. Herein, we present four critically ill patients with SARS-CoV-2 infection who received supportive care and convalescent plasma. Although all four patients (including a pregnant woman) recovered from SARS-CoV-2 infection eventually, randomized trials are needed to eliminate the effect of other treatments and investigate the safety and efficacy of convalescent plasma therapy.

The use of this file must follow the Creative Commons Attribution Non-Commercial No Derivatives License, as required by Elsevier's policy. Highlights• Barriers affecting BIM implementation in China's prefabricated construction were identified. • The interrelationships among key barriers were analyzed.• ISM methodology was applied to evaluate most influential relationships.• Recommendations for BIM implementation in China's prefabricated construction were provided.

Chromatin modification is pivotal to the epithelial-mesenchymal transition (EMT), which confers potent metastatic potential to cancer cells. Here, we report a role for the chromatin remodeling factor lymphoid-specific helicase (LSH) in nasopharyngeal carcinoma (NPC), a prevalent cancer in China. LSH expression was increased in NPC, where it was controlled by the Epstein-Barr virus-encoded protein LMP1. In NPC cells in vitro and in vivo, LSH promoted cancer progression in part by regulating expression of fumarate hydratase (FH), a core component of the tricarboxylic acid cycle. LSH bound to the FH promoter, recruiting the epigenetic silencer factor G9a to repress FH transcription. Clinically, we found that the concentration of TCA intermediates in NPC patient sera was deregulated in the presence of LSH. RNAi-mediated silencing of FH mimicked LSH overexpression, establishing FH as downstream mediator of LSH effects. The TCA intermediates a-KG and citrate potentiated the malignant character of NPC cells, in part by altering IKKa-dependent EMT gene expression. In this manner, LSH furthered malignant progression of NPC by modifying cancer cell metabolism to support EMT.

Design for manufacture and assembly (DfMA) has become a buzzword amid the global resurgence of prefabrication and construction industrialization. Some argued that DfMA is hardly new, as there are concepts such as buildability, lean construction, value management, and integrated project delivery in place already. Others believe that DfMA is a new direction to future construction. This paper aims to review the development of DfMA in manufacturing and its status quo in construction, and clarify its similarities and differences to other concepts.A multi-step research method is adopted in this study: First, an analytical framework is generated; Secondly, a literature review is conducted on DfMA in general, and DfMA-like concepts in the AEC industry; The third step is to compare DfMA with related concepts. This study reveals that DfMA as a philosophy is hardly new in construction, and the empirical implementation of many DfMA guidelines has begun in the AEC industry. The findings suggested that DfMA is a new and mixed 'cocktail' of opportunities and challenges to improve construction productivity with the advancement of construction materials, production and assembly technologies, and ever-strengthened logistics and supply chain management.This study sheds light on three research directions: DfMA implementation and guidance strategies, DfMA frameworks and blueprints, and applications in cast in-situ or intermediate prefabrication construction. Our research findings provide a synopsis of DfMA research and development in construction. This paper can also serve as a point of departure for future theoretical and empirical explorations.

BackgroundAldo-keto reductase family 1, member B10 (AKR1B10), is known to be significantly induced in the cells of various cancers such as breast cancer. However, the mechanisms of AKR1B10 promoting tumorigenesis in breast cancer remain unclear. In the present study, we demonstrated the potential role and mechanism of AKR1B10 in the invasion and migration of breast cancer cells.MethodsThe expression level of AKR1B10 in breast carcinoma, para-carcinoma and cancer tissues were detected by immunohistochemical evaluation and real-time polymerase chain reaction (RT-PCR), and the correlationships between AKR1B10 expression and clinicopathological features in breast cancer patients (n=131) were investigated. AKR1B10 was ectopically expressed in MCF-7 cells or silenced in BT-20 cells. The roles of AKR1B10 expression in the migration and invasion of MCF-7 cells and BT-20 cells were explored by wound healing assay, transwell migration assay and transwell matrigel invasion assay, and finally the activation level of extracellular signal-regulated kinase 1/2 (EKR1/2) activation and the expression level of matrix metalloproteinase-2 (MMP2) and vimentin in MCF-7 and BT-20 cells were measured by western blot.ResultsWe found that AKR1B10 expression was increased in malignant tissues, which was correlated positively with tumor size, lymph node metastasis (p<0.05). MCF-7/AKR1B10 cells displayed a higher ability of migration (43.57±1.04%) compared with MCF-7/vector cells (29.12±1.34%) in wound healing assay, and the migrated cell number of MCF-7/AKR1B10 was more (418.43±9.62) than that of MCF-7/vector (222.43±17.75) in transwell migration assay without matrigel. We furtherly confirmed MCF-7/AKR1B10 cells invaded faster compared with MCF-7/vector cells by transwell matrigel invasion assay. Finally, we found AKR1B10 induced the migration and invasion of MCF-7 and BT-20 cells by activating EKR signaling, which promoted the expressions of MMP2 and vimentin. PD98059, a specific inhibitor of the activation of MEK, blocked the migration and invasion by inhibiting the expression of MMP2 and vimentin.ConclusionsAKR1B10 is overexpressed in breast cancer, and promotes the migration and invasion of MCF-7 and BT-20 cells by activating ERK signaling pathway.

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We present a simple in situ sodium borohydride (NaBH4) reduction approach to produce amorphous core–shell like cobalt borate nanosheet-coated cobalt boride hybrid (Co-B@Co-Bi). Benefiting from the unique rich amorphous Co-Bi layer, it exhibits extraordinary catalytic activity and good stability toward the oxygen evolution reaction (OER) in 1.0 M KOH, with an overpotential of 291 mV to obtain a current density of 10 mA cm–2, outperforming most of the documented metal–metalloid-based electrocatalysts. This reverse addition strategy might provide a promising pathway for designing various transition metal borate and boride hybrid for more applications.

The pursuit of modern product sophistication and production efficiency has bolstered Design for Manufacture and Assembly (DfMA) around the world. Being both a design philosophy and a methodology, DfMA has existed in manufacturing for decades. It is coming into vogue in construction as a potential solution to the industry's lackluster productivity amid enduring exhortation of cross-sectoral learning. However, many studies of DfMA in construction are still simply following the DfMA guidelines developed from manufacturing without adequately considering important differences between the two sectors of construction and manufacturing. This study aims to develop a series of construction-oriented DfMA guidelines by adopting a mixed-method approach. It critiques existing DfMA guidelines in relation to the characteristics of construction, and further argues that construction-oriented DfMA should consider five fundamental aspects: contextual basis, technology rationalization, logistics optimization, component integration, and material-lightening, either individually or collectively. A case study is then conducted to substantiate and verify the feasibility of these guidelines. This research sheds new light on the cross-sectoral learning of DfMA from manufacturing to construction.The guidelines can be used as the benchmark for the evaluation of manufacturability and