Background Alopecia areata is considered to be an autoimmune disease characterized by T‐cell response and production of cytokines around the anagen stage. Aim of the study The aim of this study was to assess T cells in blood using immune biomarkers (CD3, CD4, and CD8) in patients with AA and AU compared with healthy individuals. Materials and methods This study was carried out at private clinic and in the Department of Dermatology in Al Karama Teaching Hospital. The study groups included 58 patients from different geographic areas in Iraq, with ages ranging from 5 to 48 years from the end of August 2019 to the January 2020; AA patient group included 19, AU patient group included, 11 and control group included 30 with no scalp or body lesions in their disease history. All patients were clinically diagnosed to detect clinical types of disease in the scalp, in the body, and any hair‐bearing area. Venous blood samples were collected from all participants where CD3 T cell, CD4 T cell, and CD8 T cell in whole blood were estimated by flow cytometry technique. Results The present study has found the males are more susceptible to infection than the females in both types of disease. There was no significant difference (p > 0.05) detected for immune biomarkers (CD3, CD4, and CD8) in blood between patient groups in comparison with healthy individuals, but there was a significant decrease (p ≤ 0.05) in immune biomarkers (CD8, CD3) according to stress status in AU and in CD8 T cell between patient groups. CD4/CD8 ratio increased in alopecia patients. Conclusion In current study, There was no significant difference detected for biomarkers (CD3, CD4, and CD8) in blood between patient groups and healthy control, but significantly decrease in CD8 T cell and CD3T cell according to stress in AU, and in CD8T cell between patient groups according to stress This research may have proven one of the theories. Study of CD markers receptors and B cell receptors these will help for future studies.
Allergic Rhinitis (AR) is a chronic immune system inflammation that occurs when the body overreacts to antigens in the environment (triggers) and produces a variety of symptoms in the nasal mucosa and paranasal sinuses due to the release of many interleukins and cytokines. We investigated the influence of the allergen on serum levels of TNF-α and Total IgE in patients with AR and their function in initiating allergic rhinitis in Iraqi petrol station workers in Baghdad. One hundred individuals with allergic rhinitis and thirty healthy workers between the ages of 20 and 59 were examined. For immunological testing, sandwich ELISA was used to evaluate serum levels of TNF-α and Total IgE. A complete blood cell count (CBC) was used for hematological testing. Findings showed a positive connection between TNF-α and Total IgE, with a correlation of (r=0.32). The TNF-α binary logistic regression analysis (BLR) was conducted to be highly significant (B = 0.02, OR = 1.02, p =0.065), viewing that an increase of one unit in TNF-α increases the odds of belonging to the AR group. TNF-α had the highest area under the curve (AUC) compared to other markers, with a value of (AUC = 0.995), indicating that it is an excellent predictor and strong marker for AR, with a sensitivity and specificity of 94% and 100%, respectively. After our research, we reached the significance of the study from the clarity of the role of tumor necrosis factor-alpha in the disease and its development and the coincidence of its increase with immunoglobulin E and considering. It is also a diagnostic marker of the disease.
A skin condition known as chronic spontaneous urticaria (CSU), which lacks an obvious triggering factor, is characterized by recurrent transient wheals, angioedema, or both for longer than six weeks. the current study was focused to study compared and diagnosis CSU by using heamatological and immunological parameters as (White blood cell count, eosinophils (EO), IL-6 and Total-IgE, in Chronic Urticaria patients were Clinically diagnosed by dermatologists in Specialized Center of Allergy in Baghdad/Al-Rusafa with age ranged between (11-60), as well as a control group included 40 with age ranged between (11-60). The results of demographic and clinical characteristics displayed the rate of female patients was higher than male which represented by 56.3 and 43.8% respectively. The results showed there was highly significant differences between the patients and the control in WBC , Total-IgE ,IL-6 the results showed EO was higher than control but no significant difference at p ≤ 0.05. The eosinophils percentage was high in age 51-60 and 21-30 year while those with age 41-50 and 51-60 year had highest, Total-IgE level 211.7 ±38.96 IU/ml, 211.69 ±38.71IU/ml respectively but IL-6 level was highest 26.4 ±1.77 pg/ml in patients with age group 21-30 year. WBC count in age 41-50 and 51-60 year showed highest count 9.2 ±1.34 cell/cm³blood, 9.3±1.21 cell/cm³blood respectively. In same time the values of parameters were various between patients without treatment and in patients who undergoes into treatment, Finally The ROC showed the IL-6 and Total-IgE were discriminated as accurate biomarkers for CSU.
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