To investigate a non-RI test which is equivalent to the reverse transcriptase inhibiting antibody test, a reverse transcriptase inhibiting antibody was compared to the absolute number of CD-4 or CD-8 cells or CD-4/-8 ratio and also to photodensitometric analysis for western blotting. There is no correlation of the reverse transcriptase with any test for cell numbers and their ratio. In photodensitometry, relative units of anti-p65 and anti-p51 were compared with reverse transcriptase inhibiting antibody. The reverse transcriptase inhibiting antibody showed a higher correlation to the relative unit of p65 antibodies than that of p51 antibodies. The photodensitometric analysis of western blotting for a serum test may be a possible method to find a prognostic marker in HIV-1 infection.
Sensitive reverse transcriptase assay was applied to human immunodeficiency virus type 2. The kinetics of this assay, stability of the enzyme and the effect of BSA to this assay indicated that the condition of this assay should be 37 degrees C in reaction temperature. The sensitivity of this assay increased by adding more than 10 micrograms/ml of BSA. The sensitivity of this assay is at least four times more than that of CPE assay using Molt-4 cell. Other HIV-2 isolate, LAV-2 in culture medium was also detectable in this condition. Moreover reverse transcriptase inhibiting antibody that specifically inhibits HIV-2 reverse transcriptase was found by this assay.
Introduction:
There are a huge number of patients who have been treated by percutaneous coronary intervention (PCI) with stenting. For PCI two types of stent are used commonly; bare-metal stent and drug-eluting stent. Although choice of the stent has high clinical importance, the information is usually hard to obtain because of onset of unexpected pathological events in emergency cases, the needs to concentrate on treatment of urgent disorders resulting in not enough attention paid to the information itself, and so on. The lack of the information may, however, lead to inadequate decision, i.e. interruption of antiplatelet therapy. Many studies have reported that cessation of antiplatelet medications produces in-stent thrombosis in patients, especially those with drug-eluting stent during early post-operative period. This complication might lead to a lethal condition in clinical practice.
Hypothesis:
Smartphone based ubiquitous system of recording coronary stent information would be useful in managing patients, who underwent percutaneous coronary intervention.
Methods and Results:
We developed a smartphone application archiving PCI data. The operation date and type of coronary stent are recorded in the memory of smartphone. The information will enable an optimal decision about continuation or discontinuation of medications including antiplatelet in the emergency setting. The time-series record about of PCI data can be accessed anytime and anywhere in the world. Feasibility and usefulness were evaluated by both volunteer patients and cardiologits. Eighty-six percent of PCI patients satisfied with and the 80% of cardiologists espoused this application.
Conclusions:
Coronary stent data recording system using smartphone technology is a simple and valuable method in managing coronary artery disease patients after interventional stent deployment. This ubiquitous application would improve health-care quality of patients who have undergone PCI.
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