No abstract

The present study investigated the effects of supplementing a low protein ( LP ) diet supplemented with key essential amino acids ( AA ) to broilers on growth performance, intestinal tract function, blood metabolites, and nitrogen excretion when the animals were maintained under various sanitary conditions for 35 D after hatching. Three hundred eighty-four one-day-old male broilers (Ross 308) were randomly allotted to groups that received one of 6 dietary treatments in a 2 × 3 factorial arrangement (i.e., 2 environmental conditions and 3 dietary treatments) to give 8 replicates per treatment. Broilers were challenged with 2 environmental conditions (sanitary vs. poor sanitary). The dietary treatments were (1) high protein ( HP ) diet, (2) LP diet, and (3) LP diet with synthetic key essential AA ( LPA ): the LP diet was supplemented with synthetic AA up to the required levels for broilers. On day 14, birds consumed the LP diet impaired growth performance compared with those fed the HP diet, while the average daily weight gain-to-feed conversion ratio of birds fed the LPA diet improved to the level of birds fed the HP diet under poor sanitary conditions ( P < 0.05). Broilers raised under poor sanitary conditions and fed the LP diet displayed higher ( P < 0.05) zonula occludens (ZO-1) expression on day 14 than broilers fed either the HP or LPA diet. Under sanitary conditions, birds fed HP and LPA diets showed higher villus height and crypt depth compared with those of broilers fed the LP diet on day 35. Moreover, broilers raised in the poor sanitary environment had higher ( P < 0.05) serum endotoxins than those raised in the sanitary environment. Broilers fed the LPA diet showed reduced ( P < 0.05) nitrogen excretion on days 14 and 35 compared with those fed the LP and HP diets independent of the environment. In conclusion, the LPA diet did not impair growth performance under poor sanitary conditions for 14 D after hatch while resulting in lower nitrogen excretion in any environment conditions throughout the experiment.

2017)R educing the dietary om ega-6 to om ega-3 polyunsaturated fatty acid ratio attenuated inflamm atory indices and sustained epithelial tight junction integrity in w eaner pigs housed in a poor sanitation condition. Animal Feed Science and Technology, 234 . pp. 312-320. Reducing the dietary omega-6 to omega-3 polyunsaturated fatty acid ratio attenuated inflammatory indices and sustained epithelial tight junction integrity in weaner pigs housed in a poor sanitation condition.Animal Feed Science and Technology http://dx.(J. M. Heo) †These authors have contributed equally to this work Highlights  A total of 108 male pigs [Duroc × (Yorkshire × Landrace); initial BW 7.1 ± 0.5 kg] weaned at 21 days of age were randomly allocated to one of 3 dietary treatments and 2 environmental conditions (sanitary vs. unsanitary) (6 replicate pens per treatment, 3 pigs per pen)  One pig per pen (n = 6) was euthanized on d 0, d 7 and d 14, to collect blood and small intestinal tissue samples  Housing pigs in an unsanitary environment after weaning increased inflammatory responses and reduced growth performance, but reducing the n-6:n-3 PUFA ratio to 4:1 attenuated the inflammatory responses induced by the unsanitary environment. ABSTRACTThe present study was conducted to determine the effect of reducing dietary n-6:n-3 PUFA ratio on the performance, inflammatory response and gut morphology of PWD challenged with sanitary and poor sanitary conditions in weaned pigs, and to test the hypotheses that (1) exposure to an poor sanitary environment will increase indices for Abbreviations: ADFI, average daily feed intake; ADG, average daily gain; ANOVA, analysis of variance; BW, body weight; COX-2, cyclooxygenase-2; CP, crude protein; D, dietary treatments; DE, digestible energy; DHA, docosahexaenoic acid; DM, dry matter; E, environmental condition (sanitary vs poor sanitary); ELISA, enzyme linked immunosorbent assay; EPA, eicosapentaenoic acid; FCR, feed conversion ratio; GE, gross energy; IL-1β, interleukin 1β; LTB4, leukotriene B4; N, nitrogen; n-6:n-3, omega-6 to omega-3; PGE2, prostaglandin E2; PPARγ, peroxisome proliferator-activated receptor-γ; PUFA, polyunsaturated fatty acid; PWD, post-weaning diarrhoea; SEM, standard error of mean; SID, standardised ileal digestible; TNF-α, tumor necrosis factor α; V:C, villous height: crypt depth.

Objective: To evaluate calcium stearoyl-2 lactylate (CSL) performance as an exogenous emulsifier together with lipase for broiler diets.Methods: In total, 252 one-day-old Ross 308 broiler chickens were allocated in a completely randomized design to give 6 replications per treatment with 7 birds in each cage. There were six dietary treatments representing a 2×3 factorial arrangement consisted of two energy levels (standard energy [positive control, PC] and –100 kcal/kg of the requirement level [negative control, NC]) and three dietary treatments (without additives [CON], CON+CSL [CSL], and CON+CSL+lipase [CSL-Lipase]). Corn and soybean meal-based experimental diets containing vegetable oil were formulated. Growth performance, blood parameters, visceral organ weights, ileal morphology, nutrient digestibility, and cytokine gene expression were measured.Results: Birds fed a diet including CSL increased (p<0.05) lipase level in blood compared to birds fed a diet including CSL-Lipase on day 21. Similarly, higher (p<0.05) liver weight was observed in birds fed a diet including either CSL or CSL-Lipase on day 21. Birds fed NC diet with CSL improved (p<0.05) nutrient digestibility compared to the NC diet on day 21. However, birds fed a diet supplemented with CSL or CSL-Lipase did not affect (p>0.05) the weight gain, feed efficiency, ileal morphology, and cytokine concentrations during the experiment period, regardless of dietary energy levels.Conclusion: Our results indicated that CSL has a role in improving nutrient digestibility in young birds when supplemented to a corn-soybean meal based broiler diet.

Objective: An experiment was conducted to investigate the response of laying hens fed corn distiller' s dried grains with solubles (DDGS) that are naturally contaminated with deoxynivalenol (DON). Methods: One hundred and sixty 52-week-old Lohmann Brown Lite hens were randomly allotted to five dietary treatments with 8 replicates per treatment. The dietary treatments were formulated to provide a range of corn DDGS contaminated with DON from 0% to 20% (i.e., 5% scale of increment). All laying hens were subjected to the same management practices in a controlled environment. Body weight, feed intake and egg production were measured biweekly for the entire 8-week experiment. The egg quality was measured biweekly for 8 weeks. On weeks 4 and 8, visceral organ weights, blood metabolites, intestinal morphology, and blood cytokine concentrations were measured. Results: The inclusion of corn DDGS contaminated with DON in the diet did not alter (p> 0.05) the body weight, feed intake, hen-day egg production, egg mass and feed efficiency of the laying hens. No difference was found (p>0.05) in the egg quality of hens that were fed the dietary treatments. Furthermore, hens that were fed a diet containing corn DDGS contaminated with DON showed no change (p>0.05) in the visceral organ weights, the blood metabolites, and the cytokine concentrations. The crypt depth increased (p<0.05) as the amount of corn DDGS contaminated with DON increased. Proportionately, the villus height to crypt depth ratio of the laying hens decreased (p<0.05) with the increasing level of corn DDGS contaminated with DON in the diet. Conclusion:The inclusion of corn DDGS contaminated with DON up to 20% in layer diets did not cause changes in egg production performance and egg quality, which indicates that DON is less toxic at the concentration of 1.00 mg DON/kg.

1. A dose-response experiment was conducted with male Korean native ducklings (KND) to evaluate the total sulphur amino acid (TSAA) requirement from hatch to 21 d of age. 2. A completely randomised design with 7 dietary TSAA concentrations (0.62%, 0.65%, 0.68%, 0.71%, 0.74%, 0.77% and 0.80%) were used with 6 replications per treatment. 3. Body weight (BW) and feed intake were measured weekly to calculate average daily gain (ADG), average daily feed intake (ADFI) and feed conversion ratio (FCR). One duckling per pen (n = 6) was killed by cervical dislocation to weigh empty body and drumsticks at the conclusion of experiment. 4. BW was improved significantly with increasing TSAA content, in a non-linear manner. A significant decrease of FCR was shown with increasing TSAA contents. TSAA requirement was determined by taking a mean value after fitting the data to both a linear-plateau and a quadratic-plateau model. Estimated TSAA requirements were 0.70%, 0.70%, 0.66% and 0.70% for the maximum BW, ADG and ADFI and for the minimum FCR, respectively. Increasing TSAA content improved quantity of full body weight (FBW), empty body weight (EBW) and drumstick weight (DSW), but there was no effect on proportion of DSW in relation to EBW and proportion of EBW to FBW. 5. In conclusion, the growth of male KND during 1 to 21 d of age was improved with increasing TSAA content, suggesting optimal TSAA requirements estimated by two analysis models.

The production performance of broiler breeder hens in response to different levels of total lysine during the early laying period was investigated. A total of 126 Ross 308 parent stock hens were offered one of seven dietary treatments formulating elevated contents of total lysine ranging from 0.55% to 0.79% (0.04 scale; 133 g of feed) from 23 to 29 weeks of age. Each treatment had six replicates with three birds per pen. Body weight was recorded triweekly and eggs were collected and weighted at 9:00 am daily. One hen from each pen was euthanized to collect blood samples and visceral organs were harvested and weighed. Egg production, egg weight and egg mass were lower ( p < 0.05) in hens offered a diet containing 0.55% total lysine compared to those fed the diet containing higher total lysine. Hens offered a diet containing 0.71%, 0.75%, and 0.79% total lysine had greater ( p = 0.008) egg production rate compared to those offered a diet containing lysine less than 0.71%. The number of total eggs produced tended to be greater ( p = 0.083) in hens offered a diet containing 0.71 and 0.75% total lysine compared to the other treatments. The number of settable egg production was higher ( p < 0.001) in hens offered a diet contacting 0.79% total lysine compared to those fed the diet containing lower levels of total lysine. The relative weights of oviduct and ovary were lower ( p < 0.05) in hens offered a diet containing 0.59% total lysine compared to the other treatments. No difference found in body weight, the number of total eggs, double-yolk eggs and abnormal shell eggs among the treatments. The urea nitrogen, estradiol-17 beta and progesterone in plasma were not affected by treatments. Based on linear- and quadratic-plateau models, total lysine requirements for egg production, settable egg production and egg mass at the early laying period were to be 0.73%, 0.77%, and 0.71%, respectively. Modern broiler breeder hens likely require higher total lysine than NRC recommendation in a diet for enhancing productivity during the early-laying period.

This study examined the effects of dietary processed pine needle tree powder (PPNP) on growth performance, visceral organ parameters, ileal digestibility, blood metabolites and ileal morphology of broiler chickens for 5-week after hatch. In total, 220 1-day-old male Ross 308 broiler chicks were randomly allocated to 30 pens (seven birds per pen), and each pen was assigned to one of five dietary treatments, a diet without PPNP (control) and diets with 0.5%, 1.0%, 1.5% and 2.0% PPNP. The body weight and feed intake were measured weekly. One bird from each pen was selected randomly and gently euthanized to collect the blood, visceral organs and digesta samples on 2, 3 and 5 week of age. Birds fed 2.0% PPNP showed decreased (P<0.05) overall body weight, average daily gain and average daily feed intake compared to those fed control, 0.5 and 1.0% PPNP. The small intestine length increased (P<0.05) as the level of PPNP in the diet increased. A deeper crypt depth was observed (P<0.05) when birds fed 2.0% PPNP compared to those fed the control diet at week 2. A higher V:C ratio was observed (P<0.05) in birds fed 1.0% PPNP than in those fed other treatments at week 3. Ileal protein digestibility improved (P<0.05) when birds fed 0.5% and 1.0% PPNP compared to that in those fed the control diet at week 3. The feed conversion ratio, ileal dry matter digestibility and blood urea nitrogen and cholesterol, and gizzard and ceca weights were not affected (P>0.05) by supplementation of any levels of PPNP. These results demonstrated that inclusion of PPNP in broiler diets could be a possible factor in improving overall growth performance suggesting early gut development when 1% PPNP is supplemented.