Aspergillus oryzae biomass grown on effluents produced during the extraction of palm oil was found to have a crude protein content of 39.6 g 100 8-1 and a 'true protein' content of 32.1 g 100 g-l. Amino acid analysis showed that the essential amino acid content was 58.2 g 16 g-' N and the essential amino acid index was calculated to be 87.4. The sulphur-containing amino acids were present at a combined level of 2.8 g 16 g-l. In rat feeding trials the biomass had a biological value of 0.68 0.03, a net protein utilisation of 0.65 2 0.05 and a true digestibility of 0.96 k 0.05 based on its crude protein content. No unusual fatty acids were detected in the biomass.
The spent wash produced by an Irish malt whiskey distillery was sampled over a four-year period. Total soluble copper levels of up to 8-5 ppm were found; these varied with wash pH. No more than 6% of total soluble copper was present as the free ion; the level varied with wash pH as well as with its free ami no acid and total soluble copper content. However, up to 83% of total spent wash copper was in an insoluble form associated with its suspended solids.
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