In a search for a 99m Tc-labelled tracer agent for imaging of amyloid plaques in patients with systemic amyloidosis (SA), we have conjugated three bifunctional chelating ligands, namely S-benzyl-mercaptoacetyl-l-aspartyl(tBu)-glycine, HYdrazinoNICotinic acid and nitrilotriacetic acid, to the 2-phenylbenzothiazole core of thioflavin-T (ThT), which has known affinity for amyloid. The compounds were successfully synthesized and labelled with technetium-99m. Their structure was confirmed by radio-LC-MS analysis. After i.v. injection in normal mice, all three 99m Tc-labelled ThT derivatives were excreted almost exclusively via liver and intestines. This indicates that the new tracer agents have not the required biokinetics for application as a probe for detection of SA in the abdominal region.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.