Tobacco streak virus (TSV), a member of the genus Ilarvirus (family Bromoviridae), has a tripartite genome and forms quasi-isometric virions. All three viral capsids, encapsidating RNA 1, RNA 2 or RNA 3 and subgenomic RNA 4, are constituted of a single species of coat protein (CP). Formation of virus-like particles (VLPs) could be observed when the TSV CP gene was cloned and the recombinant CP (rCP) was expressed in E. coli. TSV VLPs were found to be stabilized by Zn(2+) ions and could be disassembled in the presence of 500 mM CaCl2. Mutational analysis corroborated previous studies that showed that an N-terminal arginine-rich motif was crucial for RNA binding; however, the results presented here demonstrate that the presence of RNA is not a prerequisite for assembly of TSV VLPs. Instead, the N-terminal region containing the zinc finger domain preceding the arginine-rich motif is essential for assembly of these VLPs.
The homogeneous ternary cubic equation given by () 2 3 3 2 3 zw y x = + is analysed for its non-zero distinct integer solutions. A few interesting relations between the solutions and special polygonal and pyramidal numbers are presented.
Abstract. Non-homogeneous binary quadratic equation represents hyperbola given by 5 6 5 2 2 = − y x is analyzed for its non-zero distinct integer solutions. A few interesting relation between the solution of the given hyperbola, integer solutions for other choices of hyperbola and parabola are obtained.
The Binary quadratic negative pell equation 16 80 2 2 − = x y representing a hyperbola is analyzed for its non-zero integer solutions. A few interesting relations among its solutions are presented. Further, employing the solutions of the above equation, we have obtained solutions of other choices of hyperbolas, parabolas and special pythogorean triangles.
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