Despite their crucial role in health and disease, our knowledge of immune cells within human tissues, in contrast to those circulating in the blood, remains limited. Here, we surveyed the immune compartment of lymphoid and non-lymphoid tissues of six adult donors by single-cell RNA sequencing, including alpha beta T-cell receptor (αβ TCR), gamma delta (γδ) TCR and B-cell receptor (BCR) variable regions. To aid systematic cell type identification we developed CellTypist, a tool for automated and accurate cell type annotation. Using this approach combined with manual curation, we determined the tissue distribution of finely phenotyped immune cell types and cell states. This revealed tissue-specific features within cell subsets, such as a subtype of activated dendritic cells in the airways expressing CSF2RA, GPR157, CRLF2, ITGAD-expressing γδ T cells in spleen and liver, and ITGAX+ splenic memory B cells. Single cell paired chain TCR analysis revealed cell type-specific biases in VDJ usage, and BCR analysis revealed characteristic patterns of somatic hypermutation and isotype usage in plasma and memory B cell subsets. In summary, our multi-tissue approach lays the foundation for identifying highly resolved immune cell types by leveraging a common reference dataset, tissue-integrated expression analysis and antigen receptor sequencing.
In view of the complex asymmetric deformation characteristics of inclined coal seam roadways and the tensed shear failure of anchor cable supports, the asymmetric support scheme of an anchor cable with a C-shaped tube is proposed. In order to study its supporting effect on an inclined coal seam roadway, this paper first explores the difference in shear performance between an anchor cable with a C-shaped tube and an anchor cable through double shear tests. Then, based on the asymmetric deformation characteristics of an inclined coal seam roadway in the Pangpangta Mine, a numerical simulation is used to study the asymmetric support effect of an anchor cable with a C-shaped tube in an inclined coal seam roadway. The results of the double shear test show that the anchor cable with the C-shaped tube has stronger resistance to shear load than that of the anchor cable. Through the results of the numerical simulation, the original stress field distribution on both sides of the roadway was found to be uneven due to the influence of the coal seam dip angle, and after the excavation of the inclined coal seam roadway, the displacement and plastic zone distribution on both sides showed obvious asymmetric characteristics. Compared with the symmetric support, the asymmetric support can obviously alleviate the asymmetric deformation characteristics of the two sides and effectively control the deformation and plastic failure zone of the roadway. The anchor cable with the C-shaped tube has better resistance to shear deformation than that of the anchor cable. The anchor cable with the C-shaped tube can reduce the deformation and plastic area of the roadway more effectively.
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