The effects of magnetic field (MF) treatments of soybean seeds on calli growth, cell damage, and biochemical changes under salt stress were investigated under controlled conditions. Soybean seeds were exposed to a 1.0 Hz sinusoidal uniform pulsed magnetic field (PMF) of 1.5 µT for 5 h/day for 20 days. Non-treated seeds were considered as controls. For callus regeneration, the embryonic axis explants were taken from seeds and inoculated in a saline medium with a concentration of 10 mM NaCl for calli growth analysis and biochemical changes. The combined treatment of MF and salt stress was found to significantly increase calli fresh weight, total soluble sugar, total protein, and total phenol contents, but it decreased the ascorbic acid, lipid peroxidation, and catalase activity of calli from magnetically exposed seeds compared to the control calli. PMF treatment significantly improved calli tolerance to salt stress in terms of an increase in flavonoid, flavone, flavonole, alkaloid, saponin, total polyphenol, genistein, and daidzein contents under salt stress. The results suggest that PMF treatment of soybean seeds has the potential to counteract the adverse effects of salt stress on calli growth by improving primary and secondary metabolites under salt stress conditions.

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ive mungbean [Vigna radiata (L.) Wilczek] lines (CN9-5, EC693363, Harsha, KPS-1 and NM 94) were subjected to three different Extremely Low Frequency (ELF) sinusoidal magnetic field (SMF) treatments. Fresh seed lots were exposed to ELF-SMF for 5 hours/day for a total duration of 15 days. Three treatment combinations of ELF-SMF chosen for assessment and comparison were: T1-10Hz, 1500 + 250 nanoTesla (nT), T2- 50Hz, 1500 + 250nT and T3 - 100Hz, 1500 + 250nT. Non-treated seeds were maintained as control (T4). Minerals (iron, calcium, zinc and total phosphorus) and protein contents were determined in both the control and test seed lots and sprout samples raised from them. The sprouting parameters were also recorded. All three ELF-SMF treatments were significant for calcium and total phosphorus content in the test seed samples when compared to control. In the case of sprouts, with respect to minerals, all the ELF-SMF treatments were significant for calcium content when compared to control. With respect to protein, sprouts from EC 693363 line recorded 8.3% increase in protein content in T1 (10Hz) while sprouts from Harsha line recorded 7.2% increase in protein content in T2 (50Hz) compared to their respective controls. No treatment effects were observed for the sprouting parameters

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Effects of sinusoidal magnetic fields on chawki silkworm rearing have been studied. The experiment was conducted using a multi X Bi silkworm hybrid, PM x CSR 2. Disease-free layings were reared from hatching to cocooning and by subjecting first and second instar to three magnetic field frequencies: 0.1, 1.0, and 10 Hz at 1500 nT, pp, for six days at six hours per day. Controls were maintained simultaneously. Larval durations for both young-stage chawki (I and II) and late stage (III, IV, V) were calculated in days and hours, including the feeding and moulting periods for I to IV instars, and the feeding period for V instar up to the time of spinning. The study revealed that the magnetic exposures reduced both feeding and moulting times with no adverse effects on larval growth. The substantial reductions in time, 33 hours in instars I and II, and 64 hours in total larval duration, could be commercially important in chawki rearing, saving time, leaf consumption, and labor expenses.