Effects of pulsed magnetic field treatment of soybean seeds on calli growth, cell damage, and biochemical changes under salt stress

Radhakrishnan, Ramalingam; Leelapriya, T.; Kumari, B. D. Ranjitha

doi:10.1002/bem.21735

Cited by 35 publications

(20 citation statements)

References 56 publications

(57 reference statements)

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“…ions, but a positive effect of salt stress has been noticed on secondary metabolite accumulation in few medicinal plants (Ahire et al 2013). Salt stress increases the accumulation of secondary metabolites in the callus cultures of soybean (Radhakrishnan et al 2012). In the present study enhanced accumulation of bacoside A content (Fig.…”

Section: Discussionsupporting

confidence: 40%

The vacuolar proton pyrophosphatase gene (SbVPPase) from the Sorghum bicolor confers salt tolerance in transgenic Brahmi [Bacopa monnieri (L.) Pennell]

Ahire

Kumar

Punita

et al. 2018

Physiol Mol Biol Plants

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Plants overcome the effect of Na toxicity either by excluding Na at the plasma membrane or by sequestering them into the vacuoles. Influx of Na ions into the plant vacuoles is usually driven by H generated by vacuolar-type H-ATPase as well as vacuolar proton pyrophosphatse (VPPase). In the present study, we have developed transgenics via containing the recombinant vector pCAMBIA2300- gene. Transformants were produced using nodal explants. Transformants were confirmed by PCR and DNA blot analysis. qPCR analysis showed higher transcript levels of compared to untransformed control (UC). Higher VPPase activity was recorded in transgenics compared to UC. Under 150 mM salt stress, transgenic shoots showed enhanced Na accumulation with better biomass production, increased glycine betaine content, and total soluble sugar levels than UC. Transgenic shoots showed 2.9-3.8-folds lower levels of malondialdehyde content indicating lesser membrane damage. Increase in antioxidant enzyme activities (1.4-3.2-folds) was observed in transgenics compared to UC. Transgenics also displayed 7.3-9.0-folds enhanced accumulation of the medicinally important compound bacoside A. Increased biomass production, accumulation of Na, osmolytes (glycine betaine, sugars etc.), and elevated antioxidant enzyme activities indicate better osmotic adjustment in transgenics by compartmentalization of Na into the vacuoles under salt stress conditions. Thus, overexpression of in alleviated salt stress by sequestering Na.

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Section: Discussionsupporting

confidence: 40%

The vacuolar proton pyrophosphatase gene (SbVPPase) from the Sorghum bicolor confers salt tolerance in transgenic Brahmi [Bacopa monnieri (L.) Pennell]

Ahire

Kumar

Punita

et al. 2018

Physiol Mol Biol Plants

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“…It has been reported that the production of photosynthetic material plays an important role in crop growth and yield formation, and that the production of biomass and the physiological characteristics of crops are greatly affected by water stress (Wang et al 2019a). Extensive studies have shown changes in anatomical and morphological features, such as root architecture, leaf area, and plant height under water stress (Wang et al 2004, Radhakrishnan et al 2012. Moreover, excessive soil moisture (EM) has been shown to reduce stomatal conductance (gs), transpiration (E), photosynthetic rate (PN), and other physiological parameters of dry land crops such as corn (Zea mays L.) (Zaidi et al 2003) and winter wheat (Triticum aestivum L.) (Abid et al 2018).…”

Section: Introductionmentioning

confidence: 99%

Changes in photosynthetic and chlorophyll fluorescence characteristics of sorghum under drought and waterlogging stress

Zhang¹,

Zhu²,

Wang³

et al. 2019

Photosynt.

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Water stress is a key factor limiting sorghum growth and yield potential. This study investigated the changes in morphology, photosynthetic parameters, and fluorescence characteristics of sorghum under drought and waterlogging stress. The results indicated that these two types of water stress limited sorghum growth and led to a decrease in leaf chlorophyll (Chl), especially Chl a, which was accompanied by a decrease in net photosynthetic rate. In addition, under both types of water stress, the light-compensation point (LCP) and light-saturation point (LSP) both decreased, but the effect on these parameters was more obvious under drought. In terms of fluorescence parameters, the initial fluorescence and variable fluorescence increased under drought and waterlogging stress, while the maximum fluorescence did not change significantly, and the electron transport rate, photochemical quenching, and PSII actual quantum yield decreased. In summary, these results suggest that sorghum adapts to drought and waterlogging stress by reducing the leaf Chl a content, reducing LCP and LSP, and changing fluorescence parameters.

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“…Magnetic-treated water not only markedly increases crop nutrient uptake [19] and growth [25], but also significantly increases plant resistance to salt stress [26]. In this study, salt stress significantly (P < 0.001) reduced the shoot and root dry weight, root volume, nutrient uptake and antioxidant enzyme activity of cotton seedlings, whereas magnetic-treated water significantly alleviated the reduction of these values (P < 0.05).…”

Section: Discussionmentioning

confidence: 51%

Effects of Magnetic Water Irrigation on the Growth, N Uptake and Antioxidant Enzyme Activities of Cotton Seedlings

Gao¹,

Sun²,

Zhang³

et al. 2017

JAST-B

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A hydroponic experiment was carried out to investigate the effects of magnetic water irrigation on the growth, nutritional status and antioxidant enzyme activity of cotton seedlings. Four levels of magnetic-treated water irrigation (0, 100, 300 and 500 mT) and three levels of salt stress (0, 100 and 200 mM NaCl) were applied. Salt stress adversely affected the dry weight, nutrient uptake and antioxidant enzyme activities of cotton seedlings. Magnetic-treated water irrigation significantly increased cotton seedling dry weight. Cotton seedling dry weight increased by 14%, 22% and 29% under the treatments of 100, 300 and 500 mT magnetic water irrigation, respectively, compared with the control, at a salt stress level of 100 mM NaCl. Moreover, magnetic water irrigation improved N uptake, but did not significantly affect P and K uptake. Magnetic water irrigation significantly increased the activity of superoxide dismutase (SOD), peroxidase (POD) and the proline content compared to the control (0 mT). Irrigation with magnetic water could be a promising technique in agriculture, especially under salt stress conditions. A suitable magnetic intensity of 300 mT is recommended.

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Effects of pulsed magnetic field treatment of soybean seeds on calli growth, cell damage, and biochemical changes under salt stress

Cited by 35 publications

References 56 publications

The vacuolar proton pyrophosphatase gene (SbVPPase) from the Sorghum bicolor confers salt tolerance in transgenic Brahmi [Bacopa monnieri (L.) Pennell]

The vacuolar proton pyrophosphatase gene (SbVPPase) from the Sorghum bicolor confers salt tolerance in transgenic Brahmi [Bacopa monnieri (L.) Pennell]

Changes in photosynthetic and chlorophyll fluorescence characteristics of sorghum under drought and waterlogging stress

Effects of Magnetic Water Irrigation on the Growth, N Uptake and Antioxidant Enzyme Activities of Cotton Seedlings

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