Однако внесение дополнительного количества фосфора в агаризованную среду YEPD улучшало визуализацию реакции метахромазии. Исследование клеток на разных стадиях развития показало, что возраст культуры не влияет на метахроматическое окрашивание. Штамм S. cer evisiae УКМ Y-517 рекомендуется для проведения мониторинговых исследований в проекте «Гелиомед» как высокочувствительный штамм для исследований влияния космической погоды на микроорганизмы. Данная работа является продолжением исследования био-астрономического эффекта Чижевского-Вельховера. К л ю ч е в ы е с л о в а: дрожжи, метахромазия, волютиновые гранулы, космическая погода.
ABSTRACT. It is known that the bio-astronomical effect of Chizhevsky-Velhover is based on the cytochemical metachromatic reaction of polyphosphatecontaining volutin granules of microorganisms. As a result of long-term monitoring (2001-2015 years) of this of cytochemical index in yeasts Saccharomyces cerevisiae, it was shown that metachromatic staining of the granules possessed rhythmicity and connection with space weather. Under laboratory conditions the change of metachromasy was achieved by applying different stress factors: anaerobiosis, low temperature, acidity. Simulation of metachromatic staining in vitro by using solutions of inorganic polyphosphate with other compounds showed that this reaction was possible for the low concentrations of these polymers. The effect of the low concentrations was more expressed with increasing chain length of polyphosphate. The inhibition of the metachromasy in solution with added protein and calcium chloride indicated that this of reaction involved the presence of the protonated sites in the polymers. It is assumed that metachromatic staining of volutin granules in vivo may depend on other conditions including conformational restructuring of a polymer or sol-gel phase transitions. Thus, the question whether the metachromatic reaction of intracellular polyphosphate granules to the space weather changes is driven by a response to associated stress or despite the apparent similarity of the effects they are of a different nature remains unclear.
The research is devoted to the development of color imaging information technology, which is relevant for evaluating the results of cytochemical research in both biology and medicine. The aim of this work was to study the validity of software algorithms and integrated information technology for the qualitative and quantitative analysis of metachromasia reaction of volutin granules of Saccharomyces cerevisiae UCM Y-517 yeast. Methods. The object of this study was Saccharomyces cerevisiae UCM Y-517 yeast from the Ukrainian collection of microorganisms. The yeast was cultivated for 24 hours at 28oC. The metachromatic reaction of volutin granules was detected microscopically by the preparation of yeast cells stained with methylene blue. Digital images of cells were transferred to a specialized web resource for computer processing. Results. The algorithm of computer analysis of images included 3 steps: (1) recognition of single volutin granules, (2) assessment of the magnitude of metachromasia for each granule, and (3) integral estimation of the magnitude of metachromasia for the whole image. Information technology was developed and tested to support the daily monitoring of the metachromasia phenomenon, including obtaining the digital images, their transfer to a remote server, and automatic processing. Information technology is integrated into the web portal of the large-scale biophysical experiment “Heliomed”. Conclusions. The proposed method of computer image processing gave a quantitative and qualitative assessment in the automatic mode of metachromatic staining of volutin granules and confirmed the previously proposed classification of this phenomenon on the basis of visual examination.
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