Research of Metachromatic Reaction of Saccharomyces cerevisiae

Gromozova, E. N.; Kachur, T.L.; Voychuk, S.I.; Kharchuk, M.S.

doi:10.15407/microbiolj78.03.045

Cited by 5 publications

(8 citation statements)

References 3 publications

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“…As a result of long-term monitoring which was conducted from 2001 to 2015 years it was found that the metachromatic reaction of volutin granules of S. cerevisiae yeast had rhythmicity and connection with space weather [6]. Rhythm of metachromasy almost completely coincided with the known space periods.…”

Section: Resultsmentioning

confidence: 96%

“…This coincidence could mean conjugation of rhythms or synchronization of biological rhythms with cosmophysical variations but it has not a causal relationship [7]. Except the presence of rhythmicity statistically reliable correlation of this reaction with whole range of cosmophysical factors at the same period [6] could be the evidence of connection of metachromasy with space weather.…”

Section: Resultsmentioning

confidence: 99%

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The Reaction of Phosphorus-Containing Intracellular Inclusions to Space Weather Changes

Gromozova¹,

Kachur²,

Voychuk³

et al. 2015

OAP

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ABSTRACT. It is known that the bio-astronomical effect of Chizhevsky-Velhover is based on the cytochemical metachromatic reaction of polyphosphatecontaining volutin granules of microorganisms. As a result of long-term monitoring (2001-2015 years) of this of cytochemical index in yeasts Saccharomyces cerevisiae, it was shown that metachromatic staining of the granules possessed rhythmicity and connection with space weather. Under laboratory conditions the change of metachromasy was achieved by applying different stress factors: anaerobiosis, low temperature, acidity. Simulation of metachromatic staining in vitro by using solutions of inorganic polyphosphate with other compounds showed that this reaction was possible for the low concentrations of these polymers. The effect of the low concentrations was more expressed with increasing chain length of polyphosphate. The inhibition of the metachromasy in solution with added protein and calcium chloride indicated that this of reaction involved the presence of the protonated sites in the polymers. It is assumed that metachromatic staining of volutin granules in vivo may depend on other conditions including conformational restructuring of a polymer or sol-gel phase transitions. Thus, the question whether the metachromatic reaction of intracellular polyphosphate granules to the space weather changes is driven by a response to associated stress or despite the apparent similarity of the effects they are of a different nature remains unclear.

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Section: Resultsmentioning

confidence: 96%

Section: Resultsmentioning

confidence: 99%

The Reaction of Phosphorus-Containing Intracellular Inclusions to Space Weather Changes

Gromozova¹,

Kachur²,

Voychuk³

et al. 2015

OAP

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“…This may indicate the effect of EMF on the metabolism of phosphates. Furthermore, it is found that the age of culture does not affect the metachromasia of cells [67]. Consequently, the possible acceptor of EMF energy should be sought among the enzymes involved in such an exchange.…”

Section: Criterion 3 -Bioluminescence and Color Changes Bioluminescementioning

confidence: 99%

“…An indirect confirmation of exposure to EMF by mediation of particles that have magnetic moment may be the work [43], in which shown an inhibition of polymerase β and the rate of DNA synthesis in cell culture myeloid leukemia HL-60 in the presence of magnetic isotope ions 25 Mg 2+ or 67 Zn 2+ in the catalytic site in the magnetic field of the Earth. Under the influence of a magnetic field of 160 mT, the rate of DNA synthesis increased three to five times in the presence of magnetic isotopes.…”

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confidence: 99%

Prospects of Using Biological Test-Systems for Evaluation of Effects of Electromagnetic Fields

Marynchenko

Nizhelska²,

Shirinyan³

et al. 2019

Innov Biosyst Bioeng

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Electromagnetic fields (EMF) can occur both naturally and due to human activity. Nowadays, through the innovative communication technologies, the new sources of artificial EMFs are widely disseminated. Therefore, one needs to study the artificial EMFs and mechanisms of their influence on biosystems. The paper deals with the issues of the influence of artificial EMFs on human health, environment and biological objects. The experimental and theoretical investigations concerning an action of EMF are analyzed. The description of principles of standardization of non-specific EMFs and protection from the influence of manmade fields is presented. Possible mechanisms of EMF and electromagnetic radiation (EMR) action on biological objects, including those due to the accumulation of biogenic magnetic nanoparticles in an organism, are discussed. The aim of the research is to review state-of-the-art methods for detecting the biological effects of non-thermal non-ionizing EMF and EMR and to determine the prospects of using biological testsystems for evaluation of effects of EMFs. Hereby, the main attention is drawn to EMF sensors based on the cultures of microorganisms. The classification of existing test-systems is proposed according to criteria: 1) vitality of cells; 2) motor activity of cells; 3) bioluminescence and color changes under the influence of EMF. The need to develop simple and reliable biological indicators for various types of EMFs, especially for ultrahigh-frequency radiation in connection with the introduction of 5G communications technology, is substantiated. The necessity of standardized test-protocols for comparison of research results is emphasized. It is shown that the description of corresponding experiments should be accompanied by the following features: 1) name of the cell culture; native or modified cells; 2) physical characteristics of EMF or EMR (frequency, power, modulation, source type); 3) exposure duration of; 4) list of parameters to be investigated; 5) research methods; 6) absolute measurements, results and the relative magnitude of an effect; 7) what does the influence depend on (temperature, age of cell culture, composition of the nutrient medium); 8) inheritance of changes in cells; 9) relaxation time of the effect.

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“…and physiological and biochemical properties of the cells [5]. Ion channels in membranes, volutin granules in vacuoles and acidocalcisomes, enzyme active centers, etc., can be intracellular receptors of non-ionizing radiation of anthropogenic and natural origin [4,6,7]. In turn, the metabolism of inorganic polyphosphates, which contain high-energy bonds and are the main components of volutin granules, affects many intracellular processes, including various enzymatic processes and gene expression processes, as well as adhesion, motility, virulence, etc.…”

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confidence: 99%

Influence of the Radiofrequency Electromagnetic Field 40.68MHz on Adhesion of Saccharomyces cerevisiae Cells Deficient in Polyphosphatase PPN1 to Dental Alloys

Voychuk¹,

Gromozova²,

Ozhogan³

2020

Mikrobiol. Z.

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Adhesion is one of the most important properties of living organisms that help them to survive in a complex environment, but at the same time cause a variety of medical, industrial and economic problems. A lot of factors may affect adhesion and a variety of chemicals are used to decrease or prevent it, however, the influence of electromagnetic fields may significantly impact the efficiency of such compounds that is rarely considered in researches. The biosynthesis of specific adhesins requires energy stored in cells in the form of high-energy compounds, among which a special place belongs to polyphosphates (poly(P)). Poly(P) are structural units of the cell walls and influence surface charge as well. The deficiency in polyphosphatases, enzymes of the poly(P) metabolism, was shown to affect the adhesive properties of cells. Therefore, the aim of this work was to study the ability of the radiofrequency electromagnetic field to affect the adhesion of yeast cells to dental alloys and to evaluate the role of PPN1 polyphosphatase in the perception of electromagnetic signals and cell adhesion. Methods. Saccharomyces cerevisiae yeast cells of the wild type strains Y-517 and CRY, and the PPN1-deficient cells (strain CNX) were used in the study. The Ni-Cr (Wiron 99, Wirocer plus, Gialloy CB/N) and Co-Cr (Wirobond 280, Wironit, Gialloy PA) dental alloys prepared with three different casting methods were used. Yeast cells were treated with radi ofrequency electromagnetic fields (RF-EMF) (40.68 MHz, 30 W, 60 min, at thermostatic conditions) before their interaction with the alloys. Adhesion indexes were determined after direct contact of yeast cells with the surfaces of the alloys for 60 min at room temperature. Results. Chromium in the composition of the alloys had a negative effect on the adhesion, while niobium, carbon, and silicon, in contrast, stimulated it. Alloys prepared by the method of vacuum casting had the highest adhesion, while alloys prepared by centrifugal casting with melting by high-frequency current showed the greatest resistance to microbial adhesion. The efficiency of these factors significantly reduced in the case of pretreatment of yeast cells with RF-EMF and in case of deficiency of cells in PPN1: in both cases, yeast adhesion to all types of alloys was increased. The mechanisms that underlie such effects, may be different, and considering that cell surface charge and the hydrophobic properties were not changed, the effect of EMF may be a result of induction of "adaptive response" process within the yeast cells that increasing the resistance of the cell to the action of individual elements in the alloys, while, in the second case, it may be a consequence of changes in the extracellular polysaccharides composition, which take place it the PPN1-deficient cells. Conclusion. Thus, the efficiency of physical and chemical properties of dental alloys, which are aimed at the reduction of microbial adhesion, may be significantly reduced after RF-EMFs influence on the cells and in the case of polyphosphatase PPN...

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Research of Metachromatic Reaction of Saccharomyces cerevisiae

Cited by 5 publications

References 3 publications

The Reaction of Phosphorus-Containing Intracellular Inclusions to Space Weather Changes

The Reaction of Phosphorus-Containing Intracellular Inclusions to Space Weather Changes

Prospects of Using Biological Test-Systems for Evaluation of Effects of Electromagnetic Fields

Influence of the Radiofrequency Electromagnetic Field 40.68MHz on Adhesion of Saccharomyces cerevisiae Cells Deficient in Polyphosphatase PPN1 to Dental Alloys

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