This study was focused on the 3 H activity levels in the unique ''tritium valley'' around the Mohelno reservoir, which receives outlet cooling waters from the Dukovany nuclear power plant. Tritium activity levels above the background reference value were found in water from the reservoir and from the effluent part of the Jihlava water, in air moisture and in plant tissues tissue free water tritium (TFWT), and nonexchangeable organically bound tritium (NE-OBT). These zones were discernible that had noticeably different TFWT and NE-OBT values: (1) littoral zones, (2) slopes above the reservoir, (3) plateaus above the reservoir/river.Keywords Mohelno reservoir Á Dukovany nuclear power plant Á Tissue free water tritium Á Non-exchangeable organically bound tritium
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