Only the deproteinized DNA preparations of the simian adenovirus of the type 7 (SA 7) exhibited transforming and tumorigenic activity. The complex of the SA7 DNA with terminal protein (TP) did not exhibit either transforming or tumorigenic activity in cell cultures. In contrast to the transforming potential the infectious titers of the DNA - TP complex for the monkey kidney cells were 30-50 times higher than those of pure DNA. Cleavage of the SA7 DNA by specific endonucleases enhanced the tumorigenic potential of pure DNA, suppressed its infectivity and did not affect the lack of transformation capacity of the DNA - TP complex. The onc-gene was localized in the left terminal fragment with the minimal size 4,3x10(6)D in the case of R.Sal I. The tumorigenic activity was found to decrease with an increase in the size of the DNA fragment containing the onc-gene.
The article considers the influence of open-pit mining of diamonds on the state of Arctic ecosystems outside of the zone of the direct impact of the enterprise. To assess changes in natural complexes, a retrospective analysis was carried out on the basis of the data from social surveys of the local population using adapted methodological approaches and comparing descriptions of the biodiversity of aquatic macrophytes made in 1969-1970 and 2018. The comprehensive studies of peat soils and the laboratory modeling of the flow of the saponite into the watercourse were carried out, in order to understand the possible ways of the impact of diamond mining on natural complexes. The shallowing of the river, the change in the nature of soils in the lower reaches of the river, the crowding out a number of native species of aquatic plants by invasive macrophytes were educed. It is shown that saponite interacts with components of bog ecosystems, changes its physicochemical properties and forms intermolecular associates with dissolved organic matter. This contributes to the transport of mineral particles over long distances, to its accumulation in bottom sediments, and to the prolonged desorption, which creates favorable conditions for the introduction of invasive species.
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