Research is devoted to the study of a metal/carbon nanocomposite of copper as a stimulator of root formation during vegetative propagation of the Mock-orange (Garden jasmine) hybrid ( Philadelphus x hybridus hort.) of cultivars Komsomolec and Snezhnaja Burja, western Thuja (Northern whitecedar) of cultivar Pyramidalis Compacta ( Thuja occidentalis L. Pyramidalis Compacta). Using the example of green cuttings of ornamental crops at all concentrations of a finely dispersed suspension of metal/carbon nanocomposite of copper (TDS Me/C NC), a stimulating effect on their survival is shown. In the variants of treatment of cuttings with a NС suspension of 0.05, 0.5 and 1.0 % concentration, 68.2, 72.8 and 65.4 %% of cuttings rooted, which was 18.4, 23.0 and 15.6 %%, respectively, more than in the control (water) (НСР = 7.8).
The article presents the results of three-year studies on microclonal propagation of climbing roses «PalaisRoyal», «Camelot»and «Nahema». At the stage of proliferation the optimum concentration of cytokinin 6benzylaminopurine (6-BAP) in the Murashige and Skoog medium (MSO) for three varieties of roses was 1.0 mg/L. With an increase in the cytokinin content to 2.0 mg/l, a tendency to the proliferation coefficient increase was observed. Positive average correlation dependence of the proliferation coefficient on the size of a cutting was revealed. The use of the commercial product "Siliplant" as trace elements had a positive effect on lengthening of the microcuttings of three rose varieties. The proliferation coefficient was not significantly different from the control one. In order to prepare for the rhizogenesis stage, glucose is the best carbohydrate in the composition of the nutrient medium compared to sucrose, as evidenced by a statistically valid improvement in the quality of the cuttings. The optimal cultivation soil for the adaptation of rose seedlings is a physico-chemical-balanced soil mixture based on highmoor and transitional peat.
The stage of introducing explants into a sterile culture is difficult in the technology of clonal micropropagation of plants. The article shows the possible ways of sterilization and the introduction terms of explants of the clonal apple stocks 54-118 into in vitro culture in order to reduce planting infection and increase the yield of sterile viable explants. The best time for introducing clonal apple stocks 54-118 into sterile in vitro culture is the period of active shoot growth. Sterilization of explants with ethyl alcohol (70,0 %, 1 min) in combination with hydrogen peroxide (33,0 %) for 7 minutes and ethyl alcohol (70,0 %, 1 min) in combination with diacide (0,1 %) within 6 minutes contributed to the production of 63,0 % and 60,0 % of viable sterile explants.
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