Leishmaniases are infectious diseases with different clinical forms and prognoses, making accurate species identification particularly important. We evaluated the performance of LBF1/LBR1 L. (V.) braziliensis specific primers by PCR and compared the results with Leishmania spp. identified by monoclonal antibodies (mAbs). Of 29 L. (V.) braziliensis identified by mAbs, 16 (53.3%) were detected; and 7 (63.6%) of 11 unidentified Leishmania spp. showed the 536 bp band. 87.7% of serodeme III Leishmania isolates were identified by these primers. These results indicate a poor correlation between the two identification methods used, and also suggest the existence of genetic variability among L. (V.) braziliensis isolates from the northwest region of Paraná state.
40) detected by the immunofluorescent antibody test (IFAT) while four (2.4%) showed L. braziliensis-complex DNA by the polymerase chain reaction (PCR). Although no associations were found between IFAT or PCR results and age, sex, origin, free-roaming animals or length of residence at the address, the majority of IFAT- or PCR-positive dogs were from the urban area of the city and were allowed to roam freely beyond their neighborhood. The presence of anti-Leishmania braziliensis antibodies and L. braziliensis-complex DNA in dogs from this urban area near a native-forest park indicates the importance of following up on these dogs to confirm the ACL diagnosis.]]>
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