ROSE at EBUS FNA provides access to suitable material for molecular testing with increased yields in the form of needle washings for EGFR with FFPE materials for ALK and PDL1 testing.
Objectives
Stratified management of patients with non–small cell lung carcinoma (NSCLC) through epithelial growth factor receptor (EGFR) variant analysis has become standard clinical practice. The Idylla system is a fully compliant European in vitro diagnostics device, a fully automated platform designed to rapidly genotype formalin-fixed paraffin-embedded tissue samples. This retrospective study aims to validate the Idylla EGFR Mutation Test for use with extracted DNA from known NSCLC samples.
Methods
In this study, 20 µL of archival extracted DNA was placed directly inside the Idylla EGFR assay single-use cartridge. Idylla results were compared with the original Sanger sequencing reference method.
Results
The Idylla EGFR Mutation Test yielded valid results for all samples tested, confirming the variants identified by the reference method that lay within the Idylla target range. No false-positive cases were noted with the Idylla assay. Variant genotype reports were obtained within 150 minutes.
Conclusions
The Idylla EGFR assay is sensitive for extracted DNA and can be reliably applied to cytologic specimens, enabling its implementation as an ancillary first-line test for patients with NSCLC.
Upper urinary tract urothelial carcinomas (UTUCs) are uncommon, accounting for only 5%‐10% of urothelial carcinomas. Radical nephroureterectomy with bladder cuff excision is the standard treatment of high‐risk UTUC. Therefore, accurate preoperative diagnosis, including the use of urine cytology, is essential for appropriate patient management. This case study details a patient's pathway from haematuria to nephroureterectomy.
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