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This research is the first comprehensive analysis of the intrageneric relationships of Actiniopteris and Onychium based on original data including spore morphology, phylogeny and phylogeography. Actiniopteris and Onychium are members of the large subfamily Pteridoideae of the family Pteridaceae. The Pteridaceae family is considered one of the most taxonomically confusing families due to its representatives high level of polymorphism.         We used an interdisciplinary approach to study the “Onychium clade”: 13 taxa were studied using scanning electron microscopy (SEM) to characterize the morphology and morphometry of spores; 14 taxa were studied from a phylogenetic perspective, including character evolution and ancestral character state reconstructions; 15 taxa of “Onychium clade” were studied using herbarium data (B, P, PE, LE, VLA, TI, KYO, ALTB and TK) according to global botanical and geographical zones.                As a result of this integrated analysis, we established a deep divergence of the taxa O. tenuifrons and O. siliculosum from the main composition of the genus Onychium and the division the genus Actiniopteris into two clades: A. radiata–A. semiflabellata and A. australis–A. dimorpha. We found that representatives of Actiniopteris and Onychium had originated in a common African-Indo-Malesian area. The “Onychium clade” center of diversity is the Indian Region, which is experiencing high levels of human impact, leading to disjunctions among the studied taxa.

The genus Allium in Siberia has been revised. The materials of LE, WIR, MW, ALTB, NSK, OSBU herbarium collections, information resources on the Internet, literature data on the flora of various regions of Russia and neighboring states, as well as materials personally gathered during collecting missions have been analyzed. As a result, it was established that 62 Allium species grow in Siberia, of which 11 are endemic. The synopsis presented in the article reflects the modern system of the genus Allium. The information provided for each species includes that on the initial description, ecological confinement and geographical distribution, as well as the most common synonyms. The geographical analysis of Siberian alliums revealed the largest representation of species (18) with the South Siberian type of distribution area, which confirms one of the points of view that distinguishes this territory as a special center of species diversity of the studied genus.

Simplicillium lanosoniveum isolate SSBG2 was isolated from the diseased C. hesper collected from Schefflera octophylla in greenhouse of the South-Siberian Botanical Garden, and identified based on morphological observation and ITS region analysis. The infected plants were inoculated with conidial suspension of the isolate SSBG2 in concentrations 1.0*10 5 /mL. It was showed that after inoculation the C. hesper female adults were more vulnerable to infection. Larvae are affected to a lesser extent. The mycelium grows under the scale and cause the death of the insect. It was indicated that S. lanosoniveum had high infectivity against C. hesper. Infection symptoms appeared on day 7 after the inoculation, the infection reached the peak on day 20. Our study provides a new isolate that affects the Coccus hesperidum.

Molecular markers presented clear evidence that A. senescens subsp. glaucum is in the strict sense only distantly related to A. senescens and should be accepted at species rank. As the binomen A. glaucum refers to the A. schoenoprasum alliance, the name A. baicalense must be applied.

New data on the distribution of 40 species from the families Asteraceae, Cystopteridaceae, Dryopteridaceae, Euphorbiaceae, Poaceae, Rosaceae, and Woodsiaceae in Mongolia are presented in the article. For the first time, Centaurea cyanus, Cystopteris almaatensis, Potentilla × habievii, Potentilla salsa, and Potentilla smirnovii are indicated for the flora of the country. 19 species previously known in the flora of Mongolia are new for its separate botanical and geographical regions. Of these, 7 taxa were found for the first time in the Khuvsgul, 5 – in the Khovd, 4 – in the Gobi Altai and 3 – in the Mongolian Altai regions, 2 each – in the Khangai, Khentei, and Dzungarian Gobi, 1 – in the Middle Khalkha. New locations were registered for 19 rare and endemic species of the study region. There are narrow local national endemics: Potentilla coriacea, P. inopinata, P. laevipes, and P. schmakovii, among them. The findings are divided into three groups in the article. Comments about the range, relationships and main morphological differences are given for each species. Photographic materials of Potentilla salsa made in nature on the territory of Mongolia are demonstrated for the first time.

Аннотация. В сообщении анализируется подход для генотипирования сортов мягкой и твердой пшениц сибирской селекции. Показано, что при использовании RAF-анализа сорта твердых и мягких пшениц достоверно распределяются в отдельные кластеры с небольшим перекрытием. Однако чистота семенного материала не позволяет определять конкретные сорта достаточно точно, без использования в качестве поверочного контроля эталонных образцов.Ключевые слова: генотипирование, RAF, сорта, пшеница. Summary. The report analyzes the approach to genotyping varieties of common and durum wheat. It is shown that using of RAF-analysis 2 separate clusters of common wheat varieties and durum wheat varieties with little overlap. However, the purity of seeds doesn't allow us to determine the specific varieties for sure without the use of reference samples.Key words: genotyping, RAF, grades, Triticum.Для реализации проекта по гено ти-пированию сортов культурных и культиваров дикорастущих растений мы использовали раз-личные подходы. Секвенирование ДНК -не-достаточно удобный способ идентификации сортов растений, т. к. отличия в маркерных неко дирующих участках минимальны; к тому же, полногеномное секвенирование пока еще тре бует значительных материальных зат рат. Од ним из приемлемых способов гено типи ро-вания сортов растений является фрагментный ана лиз. Для генотипирования сортов пшеницы бы ли использованы различные варианты фраг-ментного анализа.Метод RAF (randomly amplified DNA fin gerprinting) является вариантом DAF-ме-то да (DNA amplification fingerprinting) -вос-про из водства большого количества ко рот ких фрагментов ДНК (до 1000 пар нук леотидов длиной) и ха рактеризуется более высокой надежностью, повторяемостью и чувст ви-тель ностью ДНК-мар керов. В отли чие от RAPD и DAF, RAF-ана лиз позволяет выя-вить максимально малые ко личества ампли-фи цированного фрагмента и об ладает низкой чувст вительностью к чис тоте ДНК. RAF-мар керы обычно выявляют доми нант ное наследование, но значительная часть выяв ляет и кодоминантное наследование, и мик ро са-теллитные локусы (Waldron et al., 2002).Материалы и методы. Материалом для выделения ДНК послужили образцы зерновок четырех сортов твердой яровой пшеницы: «Жем-чужина Сибири», «Памяти Янченко», «Алейская 50», «Омский Изумруд» и пяти сор тов мяг-кой яровой пшеницы: «Сибирский Альянс», «Новосибирская 15», «Алтайская 530», «Тю-менская 30» и «Сударыня» (табл.). Ранее эти же сорта мы изучали для сравнительного анализа количественного содержания ретротранспозона Ty1-copia относительно общего размера генома (Куцев и др., 2012).

This article presents the results of population-genetic analysis in four populations of Tanacetum vulgare (typical tansy) from Southern Siberia on the basis of the Randomly Amplified DNA Fingerprints (RAF). We used RAF to find certain molecular-genetic differences between the typical tansy and the northern tansy. Since the present results did not show any clear differences between the typical tansy and the northern tansy at species level, we suggest that T. vulgare includes two subspecies: the typical T. vulgare subsp. vulgare and T. vulgare subsp. boreale.