RCD1 protein confers multiple stress tolerance to plant, while playing in it developmental roles as well. After an extensive characterization of its gene in

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Generation of reactive oxidation species in response to different types of stress is a general phenomenon observed in plants. It is considered to be a molecular signaling mechanism of plants to encounter adverse effects. Radical-induced cell death 1 (rcd1 or Atrcd1) gene of Arabidopsis thaliana is a stress responsive gene known to interact with several transcription factors during different types of stress. It is predicted to provide scaffold for mediating interactions between two proteins using its WWE and RST domains. It also has an inactive PARP catalytic domain forming the Similar like rcd1 (SRO) family of plant PARPs along with its homologs. In this study a new homolog from Brassica napus genome (Bnsro1) was identified. Analysis of Bnsro1 was done to predict function on computational basis by comparison with its homolog. Bnsro1 has similarities with Atrcd1 at sequence level and contains same globular domains. It is predicted to be catalytically active as it conserves the 16 amino acids required for NAD

RCD1 is a signal transduction factor binding protein that gateways a myriad of developmental and stress-related pathways. It was first reported in the wild plant A. thaliana. Brassica napus is a cultivated member of the family Brassicaceae, in which the presence of this gene was reported. Using the homology data of these two family-related species, gene for this protein was mined within the genomes of Brassica carinata, Brassica juncea and Brassica oleracea, using sets of degenerate primers designed on homologous portions of the A. thaliana and B. napus orthologues. The newly identified sequences were then compared and studied using in-silico means and their 3D structures were modelled for having an estimate on their functions. Results demonstrate intergeneric conservation of this protein's domains on structural and functional levels. The newly found orthologues show potential to be regulated under salinity and oxidative stresses apart from being involved in several developmental stages. These homologues are in-stable in-vivo and bear motifs for binding a wide-variety of transcription factors.The structure superimposition studies suggest that these Brassica orthologues bear the WWE domains having transferase activity, the fact that can dramatically increase the survival of these agriculturally important crop plants amid the adverse environmental conditions. KEY WORDS

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