Bnsro1: A new homologue of Arabidopsis thaliana rcd1 from Brassica napus

Abstract

Generation of reactive oxidation species in response to different types of stress is a general phenomenon observed in plants. It is considered to be a molecular signaling mechanism of plants to encounter adverse effects. Radical-induced cell death 1 (rcd1 or Atrcd1) gene of Arabidopsis thaliana is a stress responsive gene known to interact with several transcription factors during different types of stress. It is predicted to provide scaffold for mediating interactions between two proteins using its WWE and RST domains. It also has an inactive PARP catalytic domain forming the Similar like rcd1 (SRO) family of plant PARPs along with its homologs. In this study a new homolog from Brassica napus genome (Bnsro1) was identified. Analysis of Bnsro1 was done to predict function on computational basis by comparison with its homolog. Bnsro1 has similarities with Atrcd1 at sequence level and contains same globular domains. It is predicted to be catalytically active as it conserves the 16 amino acids required for NAD

Identification of the Likely Orthologues of RCD1 within the Plant Family Brassicaceae

“…Where it has been found to be the most active member of SRO family. The members of this family harbour a central PARP domain and an N-terminal RST domain, while only RCD1 and its closest paralogue SRO1 bear an additional domain named WWE (Ahlfors et al 2004;Jaspers et al 2010 (Anjum et al 2015). Here we report the existence of members of the WWE bearing SRO family from B. oleracea, B. carinata and B. juncea; and make a comparative assessment of their putative products using in-silico means.…”

Genome-wide identification of SIMILAR to RCD ONE (SRO) gene family in rapeseed ( Brassica napus L.) reveals their role in drought stress response

“… 20 However, currently, only one SRO gene has been reported in rapeseed, and its study is limited to sequence analysis. 26 The number of members of the SRO gene family in rapeseed and their response to adverse stressors, especially drought stress, are unknown.…”

Isolation and Identification of Ipomoea cairica (L.) Sweet Gene IcSRO1 Encoding a SIMILAR TO RCD-ONE Protein, Which Improves Salt and Drought Tolerance in Transgenic Arabidopsis

“…Moreover, it is known that some SRO proteins hold a WWE domain in their N-terminal sequences [20,21]. Many SROs have been further investigated in various plants, including Arabidopsis [22], rice [23], wheat [24], maize [25], tomato [26], cotton [27], Brassica napus [28] and apple [29]. The first SRO protein identified from Arabidopsis thaliana is RCD1, named after its function in cell death induced by radicals.…”