The antioxidant potential of various extracts was evaluated using different antioxidant assays such as 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay, ferric reducing antioxidant power (FRAP) assay, and 2,2-azinobis-ethylbenzothiozoline-6-sulphonic acid (ABTS) using UV spectrophotometer. The highest absorbance was observed in ethanolic extracts (EEs) of Euphrasia stricta 71.92 ± 1.22%, 65.77 ± 1.38%, and 67.88 ± 0.74%, followed by methanolic extracts (MEs) 70.14 ± 0.82%, 64.84 ± 0.74%, and 65.48 ± 1.40% for DPPH assay (517 nm), FRAP assay (700 nm), and ABTS assay (734 nm), respectively. The EEs of Euphorbia platyphyllos L. showed the antioxidant activity of 69.76 ± 1.48%, 64.42 ± 0.88%, and 65.54 ± 1.36% and MEs 68.00 ± 1.50%, 62.92 ± 0.64%, and 63.42 ± 0.94% for DPPH, FRAP, and ABTS assays, respectively. So, this research suggested that these medicinal plants possess a significant antioxidant potential and are important source of natural antioxidants and can be effectively used in treating oxidative stress disorders.
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