In the present study, the antioxidant activity of successive leaf extracts of Dracaena reflexa was investigated using the scavenging activity on 1,1-diphenyl-2-picrylhydrazyl and reducing power by ferric reducing antioxidant power assay. Methanol extract was found potent in both the assays. IC50 values of 1,1-diphenyl-2-picrylhydrazyl assay for methanol extract was 0.97 mg/ml and ferric reducing antioxidant power value for the same is 1.19. Phytochemical screening, proximate analysis and total phenolic content were also determined. Qualitative screening for phytochemical showed the presence of alkaloids, flavonoids, terpenoids, glycosides and saponins. Highest phenolic content was shown by methanol extract (49.69 mg gallic acid equivalent/g dry weight). Proximate analysis showed moisture content (3.31%), ash content (8.02%), crude fibre (1.31%), crude fat (0.97%), total protein (3.70%), total carbohydrate (86.01) and nutritive value (367.56 kcal/100 g), which would make it a potential nutraceutical. This study suggested that Dracaena reflexa, a potential natural free radical scavenger, which could find use as an antioxidative.
The present work deals with the isolation of essential oil from the leaves of Casearia tomentosa as well as its chemical characterization by GC-MS and evaluation of in vitro pharmacological activities. The crude essential oil was obtained using Clevenger apparatus. Thirteen compounds were identified representing 77.62% of the total oil composition. The oil was mainly composed of 9,12-Octadecadienoic acid, ethyl ester (31.45%) followed of the 9,12,15-Octadecatrienoic acid, ethyl ester (20.11%), Phytol (10.70%), Di-epi-alpha-cedrene (3.74%), β-bisabolene (1.87%), β-Caryophyllene (0.83%) and some other trace components. Antidiabetic activity estimated by α- amylase and α- glucosidase inhibitory assay and antioxidant activity by 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay and ferric reducing antioxidant potential (FRAP) method while antibacterial activity was performed by disk diffusion method. The results demonstrated prominent α- amylase (IC50 = 184.47 ± 1.06 μg/mL), α- glucosidase (IC50 = 91.10 ± 0.80 μg/mL) inhibition, DPPH radical scavenging (IC50=75.50 ± 0.60μg/mL) and FRAP activity (37.55 ± 0.66 μM/mL, FRAP value 1.636). Antimicrobial activity performed towards the selected five human pathogenic bacterial cultures like Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumonia and Salmonella typhi. Highest inhibitory activity was seen against Staphylococcus aureus (zone of inhibition 12.2 mm) while the weakest activity was demonstrated against Pseudomonas aeruginosa (zone of inhibition 8.9 mm). Overall results revealed that C. tomentosa leaves (CTL) essential oil exhibits potent antidiabetic, antioxidant and antibacterial activities. This is the first study regarding the isolation of essential oil from the leaves of Casearia tomentosa and its pharmacological activities
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