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Context: The fruit of Terminalia chebula Retz. (Combretaceae) has been used for several therapeutic purposes in Thai folk medicines. Currently, the ethanol extracts containing antioxidant compounds have shown the ability to promote collagen synthesis.Objective: This purpose of this work was to study the effects of the ethanol extract from T. chebula fruit on the inhibition of cutaneous photodamage. Materials and methods: The viability of human skin fibroblasts after incubation with T. chebula at concentration 0.5-50 lg/mL for 24, 48 and 72 h was assessed by using sodium 3 0 -[(phenyl-amino)-carbonyl]-3,4,tetrazolium-bis(4-methoxy-6-notro)benzene-sulphonic acid hydrate (XTT). The levels of type I procollagen and matrix metalloproteinases (MMP)-1 and MMP-13 produced by UVB-irradiated fibroblasts were determined by ELISA. Skin thickness and collagen content caused by long-term UVB irradiation in male ICR mice were determined from haematoxylin and eosin stained tissue sections and spectrophotometric measurement of hydroxyproline. Results: The extract (0.5-50 lg/mL) had no effect on cell viability or morphology of the human fibroblasts. In vitro studies showed that the T. chebula extract reduced the UVB-induced MMP-1 and MMP-13 expression, whereas an increased production of type I procollagen was observed. In a UVB-irradiated animal model, male ICR mice with hair shaved were chronically exposed to UVB which lead to epidermal thickness and loss of hydroxyproline. However, these effects were fully prevented by the topical application of the T.

Objective: We compared the efficacy of an antiacne hydrogel formulated with a combination of Aloe barbadensis leaf extract, Garcinia mangostana peel extract, and Camellia sinensis leaf extract (AGC) at a ratio of 50:25:1 with a marketed 1% clindamycin gel (CG) formulation on antiacne and antiblotch activities. Methods: A single-center, parallel-arm, randomized controlled trial was performed from November 2017 to April 2018. Sixty subjects with mild–moderate acne severity according to the the American Academy of Dermatology were enrolled for the study. Outcome end points were total acne lesions (TALs) and acne-severity index (ASI) by counting the inflamed lesions and comedones and skin colors using erythema and melanin values. Results: For TALss, a decrease ( P <0.0001) in the number of total inflamed lesions from baseline was evidenced in AGC group, but not in the CG group. Higher reduction in mean ASI in the AGC group was seen than in the CG group. However, there was no statistically significant difference regarding reduction in ASI between the AGC and CG groups. For erythema, a remarked reduction in skin redness from baseline was clearly seen at day 3 ( P <0.05) in the AGC group. No significant decrease in erythema values from baseline was seen in the CG group. A significant decrease ( P =0.037) in mean melanin value from baseline was seen in the AGC group after 14 days of twice-daily use, but not in the CG group. Both products were well tolerated, with no reports of severe adverse events. Conclusion: An anti-acne hydrogel containing a combination of mangosteen rinds, aloe vera gel, and green tea–leaf extracts was superior to 1% clindamycin gel in antiacne and antiblotch activities when measured by TALs and erythema and melanin values.

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Cubosomes and other lyotropic liquid crystal (LLC) phases are of great interest in many fields due to their potential as delivery systems. This study prepared poloxamer 188 (Pluronic ® F68 [F68]) based cubosomes utilizing a low energy method and reports LLC structure stability, rheological behavior and carrier properties of the formed cubosomes. Mixtures of F68 and water were investigated and showed a concentration dependent viscoelastic behavior, ranging from a viscous liquid (30% F68) to hard gels (70% F68). Small-angle X-ray scattering showed cubosomes (Im3m) were present at F68 concentrations between 45% and 60%. Cubosomes fabricated from 50% F68 and 50% water were selected as the best composition and gave stable cubic structures for at least 10 months without additional stabilizing agent. To assess the use with poor water-soluble molecules, artocarpin (AR) was added to the F68-based cubosome. The developed cubosome possessed enhanced AR solubility due to the high affinity to the hydrophobic regions present in the cubic formation. Furthermore, an in vitro permeation study showed faster permeation rates of the developed cubosomes when compared to a cream-based formulation. Collectively, these findings demonstrate that the F68-based cubosomes represent a promising low-cost active carrier for enhancing the solubility and permeability of low water-soluble molecules.

In this study, we investigated the protective effect of hydroglycolic crude extract of Thai red Hom-Kularb-Drice ( HKD) rice bran on UVBinduced photoaging of human skin fibroblast. The HKD rice bran extract showed scavenging capacity with an EC50 of 4.37 0.34 mg/mL testing using a DPPH radical scavenging assay. The structural identification of proanthocyanidins compounds was performed by Liquid chromatographyelectrospray ionization-quadrupole-time-of flight mass spectrometry ( LC-ESI-Q-TOF-MS) in the hydroglycolic HKD extract. These compounds were cyanidin B2 type and ( epi) catechin. The extract showed a no cytotoxic response was showed in human skin fibroblast. Furthermore, the HKD extract ( 20 µg/ mL) exhibited a protective effect in UVB irradiated primary skin fibroblast, reducing MMP-1 ( matrix metalloproteinases-1) expression and increasing production of type I procollagen. This data suggests that the CMU

The aim of this study was to formulate bio-cellulose film incorporating Punica granatum (pomegranate) peel extract for application as anti-acne product. The dried pomegranate peels were extracted by water (PPEwater), 50 % (PPE50E), 70 % (PPE70E) or 95 % (PPE95E) (v/v) ethanol. The extracts were total phenolic content (TPC) and free radical scavenging activity. PPE50E had highest TPC (55.95±1.44 gGAE/100g of dried peel extract), highest free radical scavenging activity with IC50 of 18.77±1.73 µg/ml and exerted the activity of bactericidal against Staphylococcus aureus, Staphylococcus epidermidis and Propionibacterium acne (P. acne). The PPE50E was selected for formulating anti-acne bio-cellulose (BC) film. The BC film prepared from bacteria Acetobacter xylinum. It showed a strong film with the tensile strength in range of 5 - 15 MPa and the elongation at break about 5 %. The simple soaking method was used to combine BC with 5 or 10 mg/ml of PPE50E. By the SEM, the assemble nano-fibrils and the layer structure of the BC combining with the PPE50E (BCP) was found. The BCP had higher tensile strength and lower elongation at break than the BC. The BCP at wet state showed a soft and flexible film. The anti-bacterial efficacy of the BCP against tested bacteria was observed, according to the disc diffusion assay. The P. acne inhibition zones of the BCP at the concentration of 5 and 10 mg/ml were 27.7 and 35.5 mm which closed to the clindamycin (45.5 mm) as positive control. From the remarkable results, the BCP is interestingly natural device for acne treatment.

This study aimed to examine the effect of high protein from chicken breast products during resistance exercise training on muscle mass and strength in healthy Thai male volunteers. In this study was double-blind randomized placebo-controlled study. This study assessed changes in muscle mass and strength of 60 healthy men aged 20-35 years with normal BMI (23.04±2.52 kg/m2). Participants were randomly divided into three groups receiving controlled placebo tablet (CG, n=18, lactose-based containing 12.0 g lactose/day), chicken tablet (CT27, n=18, 27.0 g protein/day), and chicken chip (CC36, n=19, 36.8 g protein/day) during 69-day period. In addition, all groups were instructed to perform the same resistance training program (4 times per week). Body composition (BIA), anthropometry (AMC, CMC), and muscle strength were measured. After 9 weeks of the study, CC36 group had a statistically significant increase (p<0.05) in the percentage of skeletal muscle from baseline when compared other two groups. Additionally, a significant increase (p<0.01) in arm muscle circumference, back-leg extension, and hand grip strength were observed in both groups receiving chicken protein products. The same result was also detected in the placebo group but at a much slower rate. Resistance training exercise along with intake of the chicken protein products could increase muscle mass and strength.