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Several new platinum monolayer protected clusters (MPCs) have been synthesized and characterized. Two methods of platinum reduction were used depending on the solubility of the thiol:  sodium borohydride for the water-soluble thiols and lithium triethylborohydride for the organic soluble thiols. In general, reactant solutions containing a 1:1 thiol/Pt ratio yielded the best particles in a single-phase reaction. Higher thiol/Pt ratios produced lower yields of MPCs, while much lower ratios produced gray-black precipitates. The Pt MPCs were used as catalysts to hydrogenate allyl alcohol to propanol by reducing the carbon−carbon double bond. The Pt−mercaptoammonium MPCs were also used as catalysts in the hydrogenation of maleic acid to succinic acid. Differences in the catalytic hydrogenation rates among the various monolayer coatings for MPCs are attributed to the variations in ligand chain length, branching, charged functional groups, packing density, and core size.

A microphysiometer capable of measuring changes in extracellular glucose, lactate, oxygen, and acidification rate has been developed by incorporating modified electrodes into a standard Cytosensor Microphysiometer plunger. Glucose and lactate are measured indirectly at platinum electrodes by amperometric oxidation of hydrogen peroxide, which is produced from catalysis of glucose and lactate at films containing their respective entrapped oxidase. Oxygen is measured amperometrically at a platinum electrode coated with a Nafion film, while the acidification rate is measured potentiometrically by a Cytosensor Microphysiometer. Analytical information is obtained during the Cytosensor stop-flow cycles, where the electrodes measure changes in the extracellular medium corresponding to the consumption or production of the analyte by the cells. Modification of the Cytosensor plunger for multianalyte determination is described, and the operation of the technique is illustrated by the simultaneous measurement of all four analytes during the addition of fluoride and DNP to Chinese hamster ovary cells and fluoride and antimycin A to mouse fibroblast cells. Cell metabolic recovery and dynamics after exposure to agents can also be observed in specific cases.

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Harnessing the potential of cells as complex biosensors promises the potential to create sensitive and selective detectors for discrimination of biodefense agents. Here we present toxin detection and suggest discrimination using cells in a multianalyte microphysiometer (MMP) that is capable of simultaneously measuring flux changes in four extracellular analytes (acidification rate, glucose uptake, oxygen uptake, and lactate production) in real-time. Differential short-term cellular responses were observed between botulinum neurotoxin A and ricin toxin with neuroblastoma cells, alamethicin and anthrax protective antigen with RAW macrophages, and cholera toxin, muscarine, 2,4-dinitro-phenol, and NaF with CHO cells. These results and the post exposure dynamics and metabolic recovery observed in each case suggest the usefulness of cell-based detectors to discriminate between specific analytes and classes of compounds in a complex matrix, and furthermore to make metabolic inferences on the cellular effects of the agents. This may be particularly valuable for classifying unknown toxins.