Signals that determine the differentiation of naïve CD4
+
T helper (T
H
) cells into specific effector cell subsets are primarily stimulated by cytokines, but additional signals are required to adjust the magnitude of T
H
cell responses and set the balance between effective immunity and immunological tolerance. By inducing the post-thymic deletion of the T cell lineage signaling protein THEMIS, we showed that THEMIS promoted the development of optimal type 1 immune responses to foreign antigens but stimulated signals that favored encephalitogenic responses to self-neuroantigens. THEMIS was required to stimulate the expression of the gene encoding the transcriptional regulator T-BET and the production of the cytokine interferon-γ (IFN-γ), and it enhanced the ability of encephalitogenic CD4
+
T cells to migrate into the central nervous system. Consistently, analysis of THEMIS expression in polarized CD4
+
T cells showed that THEMIS was selectively increased in abundance in T
H
1 cells. The stimulation of predifferentiated effector CD4
+
T cells with antigen-presenting cells revealed a stimulatory function for THEMIS on type 1 cytokine responses, similar to those observed ex vivo after immunization. In contrast, THEMIS exerted opposing effects on naïve CD4
+
T cells in vitro by inhibiting the T cell receptor (TCR)–mediated signals that lead to T
H
1 cell responses. These data suggest that THEMIS exerts TCR-independent functions in effector T cells, which increase the magnitude of normal and pathogenic T
H
1 cell–mediated responses.
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