Interactions between butterflies and caterpillars in the genus Pieris and plants in the family Brassicaceae are among the best explored in the field of insect-plant biology. However, we report here for the first time that Pieris brassicae, commonly assumed to be a typical folivore, actually prefers to feed on flowers of three Brassica nigra genotypes rather than on their leaves. First-and second-instar caterpillars were observed to feed primarily on leaves, whereas late second and early third instars migrated via the small leaves of the flower branches to the flower buds and flowers. Once flower feeding began, no further leaf feeding was observed. We investigated growth rates of caterpillars having access exclusively to either leaves of flowering plants or flowers. In addition, we analyzed glucosinolate concentrations in leaves and flowers. Late-second-and early-third-instar P. brassicae caterpillars moved upward into the inflorescences of B. nigra and fed on buds and flowers until the end of the final (fifth) instar, after which they entered into the wandering stage, leaving the plant in search of a pupation site. Flower feeding sustained a significantly higher growth rate than leaf feeding. Flowers contained levels of glucosinolates up to five times higher than those of leaves. Five glucosinolates were identified: the aliphatic sinigrin, the aromatic phenyethylglucosinolate, and three indole glucosinolates: glucobrassicin, 4-methoxyglucobrassicin, and 4-hydroxyglucobrassicin. Tissue type and genotype were the most important factors affecting levels of identified glucosinolates. Sinigrin was by far the most abundant compound in all three genotypes. Sinigrin, 4-hydroxyglucobrassicin, and phenylethylglucosinolate were present at significantly higher levels in flowers than in leaves. In response to caterpillar feeding, sinigrin levels in both leaves and flowers were significantly higher than in undamaged plants, whereas 4-hydroxyglucobrassicin leaf levels were lower. Our results show that feeding on flower tissues, containing higher concentrations of glucosinolates, provides P. brassicae with a nutritional benefit in terms of higher growth rate. This preference appears to be in contrast to published negative effects of volatile glucosinolate breakdown products on the closely related Pieris rapae.

Plants attacked by herbivorous insects emit volatile compounds that attract predators or parasitoids of the herbivores. Plant fitness increases when these herbivorous insects are parasitized by solitary parasitoids, but whether gregarious koinobiont parasitoids also confer a benefit to plant fitness has been disputed. We investigated the relationship between parasitoid load of the gregarious Cotesia glomerata (L.) (Hymenoptera: Braconidae), food consumption by larvae of their host Pieris brassicae L. (Lepidoptera: Pieridae), and seed production in a host plant, Brassica nigra L. (Brassicaceae), in a greenhouse experiment. Plants damaged by caterpillars containing single parasitoid broods produced a similar amount of seeds as undamaged control plants and produced significantly more seeds than plants with unparasitized caterpillars feeding on them. Increasing the parasitoid load to levels likely resulting from superparasitization, feeding by parasitized caterpillars was significantly negatively correlated with plant seed production. Higher parasitoid brood sizes were negatively correlated with pupal weight of Cotesia glomerata , revealing scramble competition leading to a fitness trade-off for the parasitoid. Our results suggest that in this tritrophic system plant fitness is higher when the gregarious parasitoid deposits a single brood into its herbivorous host. A prediction following from these results is that plants benefit from recruiting parasitoids when superparasitization is prevented. This is supported by our previous results on down-regulation of synomone production when Brassica oleracea was fed on by parasitized caterpillars of P. brassicae . We conclude that variable parasitoid loads in gregarious koinobiont parasitoids largely explain existing controversies about the putative benefit of recruiting these parasitoids for plant reproduction.

The western conifer seed bug, Leptoglossus occidentalis Heidemann, and the southern pine seed bug, L. corculus (Say), feed on the developing seeds of conifers causing great losses to seed crops (Koerber 1963; Connelly and Schowalter 1991; Schowalter 1996). When high populations threaten the yieldof genetically superior, high quality seed in seed orchards, chemical controls may be used (DeBarr et al. 1982). However, because even minor uses of chemical pesticides are increasingly restricted, particularly in British Columbia where seed orchards commonly interface with urban areas, damaging populations of L. occidentalis may not be controlled at all.

Black mustard, Brassica nigra (L.) Koch, is a wild annual species found throughout Europe and fed on by larvae of the large cabbage-white butterfly, Pieris brassicae L. We examined the impact of herbivory from P. brassicae, a gregarious herbivore, on B. nigra grown from wild seed collected locally. In greenhouse studies, the response of B. nigra to four herbivore densities in two developmental stages of the plant was quantified by measuring leaf damage, plant height, days to flowering, silique number, and seed production. Pieris brassicae readily attacked B. nigra leaves, although the timing of the attack did not affect seed production; attacked plants produced as many seeds as as nonattacked plants. Plant height was affected when plants were attacked early, but not later, in development, suggesting a connection between their belowground zone of influence and ability to regain biomass. These results demonstrate that at the herbivore densities and timing of damage studied, B. nigra tolerates folivory from Pieris brassicae through compensation.

Secondary succession was documented throughout a long-term study of two abandoned hay fields in south-eastern Ontario. In one field ͑Field 1͒, eighteen plots ͑measuring 100 m 2 ͒ were established. Nine of these plots were ploughed to bare ground and nine remained in their original abandoned state. A third set of nine plots were established in a second field ͑Field 2͒, which had been abandoned 5 years earlier. Detailed surveys of the plant flora were conducted twice yearly, insect sweep data were collected bi-monthly, from 1976 Ϫ 1988, 1995 and 1998. Soil and climate data were collected and community patterns analyzed against these variables and field treatment ͑ploughing͒. While the vegetation in all plots began predominantly as a grass mixture, only a few exhibited the typical secondary succession pathway for this region. The remaining plots persist at various stages of succession, with some still in the early stages. Succession stage, as quantified by a succession ratio, varied across these plots largely by soil moisture, and to a lesser degree by field treatment ͑ploughing͒. When grouped by soil moisture, wet plots had lower ratios ͑characterized by later species and trees͒, than dry plots ͑characterized by early or grass species͒. Species richness was highest in plots that were wet, regardless of field treatment. Partial CC analysis using CANOCO of plant species versus field treatment, soil moisture and insect outbreak determined their contribution to the plant variation observed. It is generally accepted that plots over time, regardless of initial composition, will converge to become similar. Convergence of our plots, grouped according to soil moisture, did not occur. Analyses of insect outbreaks against changes in the succession ratio over time provide some evidence that herbivory can influence the likelihood of plots to converge.

Leptoglossus occidentalis Heidemann (Hemiptera: Coreidae) is considered to be an important cone and seed pest in seed orchards in British Columbia; however, no impact studies have been done in Canada. Surveys to determine the distribution of L. occidentalis were conducted over a 3-year period in three coastal seed orchards of Douglas-fir, Pseudotsuga menziesii (Mirb.) Franco, and over a 2-year period in two seed orchards of lodgepole pine, Pinus contorta var. latifolia Engelmann. Leptoglossus occidentalis was found to distribute itself in patches, coinciding with a clonal preference. Seed losses were assessed for 2 years in the coastal Douglas-fir orchards and for 1 year in one lodgepole pine orchard. The percentages of partially filled seed that could have been caused by feeding of L. occidentalis or by a number of other factors were < 5% in coastal Douglas-fir for both 1992 and 1993 and ~14% in lodgepole pine in 1993. Apparently because populations of L. occidentalis were low, there was no correlation between the percentage of partially filled seed and number of insects per tree. Except in unusual circumstances, management measures in Canada are judged not to be necessary as populations of L. occidentalis are consistently below the calculated threshold levels required to cause ≥5% loss in seed production.

No abstract

No abstract