A reverse phase high performance liquid chromatographic method (HPLC) has been developed for the estimation of Alfuzosin hydrochloride (ALH) in the pharmaceutical formulation using RP-C18 column. The mobile phase (Tetrahydrofuran, Acetonitrile and buffer (pH 3.50) was pumped at a flow rate of 1.5 ml/min in the ratio of 1:20:80 and the eluents were monitored at 254.0 nm. Linearity was obtained in the concentration range of 80 -120 µg/mL for ALH. The method was statistically validated and RSD was found to be less than 2% indicating high degree of accuracy and precision of the proposed HPLC method. Due to its simplicity, rapidness, high precision and accuracy, the proposed HPLC method may be used for determination of Alfuzosin hydrochloride in bulk drugs and in pharmaceutical dosage form.
This paper presents the development and validation of high performance thin layer chromatography (HPTLC) with a densiotometric detection method for the simultaneous analysis of Gliclazide and Metformin hydrochloride. A separation was performed on silica gel 60F254 plates. The mobile phase comprised of toluene, acetonitrile, ethanol, Ammonium sulphate (0.25%) (4 : 4 : 4 : 3, v/v/v/v). The detection of wavelength was found to be 228nm. The Rf values of GLZ and MET were found to be 0.69 and 0.37 respectively. The accuracy and reliability of the method was assessed by evaluation of linearity 200-1000ng per spot of GLZ and MET. Precision intra-day and inter-day RSD values were always less than accuracy (97.37 for GLZ and 94.8 for MET) specificity in accordance with ICH guidelines. The developed HPTLC method was found to be new, accurate and precise.
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