Development and Validation of New RP-HPLC Method for the Estimation of Alfuzosin Hydrochloride in Bulk and Tablet Dosage Form

Abstract

A reverse phase high performance liquid chromatographic method (HPLC) has been developed for the estimation of Alfuzosin hydrochloride (ALH) in the pharmaceutical formulation using RP-C18 column. The mobile phase (Tetrahydrofuran, Acetonitrile and buffer (pH 3.50) was pumped at a flow rate of 1.5 ml/min in the ratio of 1:20:80 and the eluents were monitored at 254.0 nm. Linearity was obtained in the concentration range of 80 -120 µg/mL for ALH. The method was statistically validated and RSD was found to be less than 2% indicating high degree of accuracy and precision of the proposed HPLC method. Due to its simplicity, rapidness, high precision and accuracy, the proposed HPLC method may be used for determination of Alfuzosin hydrochloride in bulk drugs and in pharmaceutical dosage form.

A synchronous spectrofluorometric technique for simultaneous detection of alfuzosin and tadalafil: applied to tablets and spiked biological samples

“…By reviewing the previous literature, several methods were reported for the determination of AFH and TDF. For determination of AFH, HPLC [15][16][17][18][19], spectrophotometric [20][21][22][23][24][25], spectrofluorometric [25][26][27][28] and voltammetric [29][30][31] procedures were revealed. For TDF determination, different HPLC [32][33][34], spectrofluorometric [35][36][37][38], spectrophotometric [35,[39][40][41][42] and voltammetric [43][44][45][46] methods were reported for its analytical quantitation.…”

Development and Validation for Simultaneous Estimation of Proguanil and Atovaquone by using RP-HPLC

“…Literature survey reveals that several analytical methods have been reported for Proguanil and Atovaquone individually in biological fluids and in pharmaceutical dosage forms [3][4][5][6][7][8].…”

Simultaneous Spectrophotometric Estimation of Bisoprolol Fumarate and Hydrochlorothiazide in Tablet Formulation using Partial Least-Squares, Principal Component Regression Multivariate Calibrations and RP-HPLC Methods

“…Therefore, their simultaneous determination is hard by using conventional spectrophotometric techniques. Several methods have been reported for the analysis of BS or HZ alone or in combination with other drugs [2][3][4][5][6][7][8][9][10][11][12][13][14][15] and in their binary mixture using HPLC [16][17][18][19][20][21][22][23]. Both BS and HZ are official in The United States Pharmacopeia [24] and British Pharmacopeia [25].…”