The extreme drought tolerance of the resurrection fern is in part the result of the dorsal scales that assist in water distribution and controlled desiccation. We studied the effect of peltate scales on water uptake and loss of the desiccation-tolerant epiphytic fern Pleopeltis polypodioides using optical and FTIR microscopy and staining with calcofluor, solophenyl flavine7GFE, and Ruthenium Red. We provide information on structure, property, and function of the scales by measuring water uptake and dehydration, contact angles, and metabolic activity. Peltate scales mainly contain cellulose, xylogalactans, and pectin. Water is absorbed from the center of scales, and the overlapping arrangement of scales facilitates surface spreading of water. Intact fronds hydrated fully within 5 h of imbibition of the apical pinna, without scales water uptake stopped after 1 h. Hydration rates via rhizomes followed a longer time course but also improved in the presence of scales. Fronds with and without scales lost half of their water content in 15 or 4 h, respectively. The overall metabolism of rapidly dehydrated fronds was significantly reduced compared with slowly dehydrated fronds. Thus, water management and metabolism of Pleopeltis are dependent on surface properties determined by peltate scales.
Magnetic gradients are able to move diamagnetic compounds under weightless or microgravity conditions and serve as directional stimulus during seed germination in low-gravity environments. Further work can determine whether gravity sensing is based on force or contact between amyloplasts and statocyte membrane system.
Although heat and desiccation stresses often coincide, the response to heat especially in desiccation tolerant plants is rarely studied. We subjected hydrated Pleopeltis polypodioides fronds to temperatures up to 50°C and dehydrated fronds up to 65°C for 24 h. The effect of heat stress was evaluated using morphological changes, photosystem (PS) II efficiency, and metabolic indicators. Pinnae of dried fronds exposed to more than 40°C curled tighter and became brittle compared to fronds dried at lower temperatures. Exposure to > 50°C leads to discolored fronds after rehydration. Hydrated fronds turned partially brown at > 35°C. Chlorophyll fluorescence (Ft) and quantum yield (Qy) increased following re-hydration but the recovery process after 40°C treatment lasted longer than at lower temperatures. Similarly, hydrated fronds showed reduced Qy when exposed to > 40°C. Dried and hydrated fronds remained metabolically active up to 40°C. Hydroperoxides and lipid hydroperoxides in dried samples remained high up to 50°C, but decreased in hydrated fronds at > 40°C. Catalase (CAT) and glutathione (GSH) oxidizing activities remained high up to 40°C in dehydrated fronds and up to 35°C in hydrated fronds. Major fatty acids detected in both dehydrated and hydrated fronds included palmitic (C16:0) and stearic (C18:0) acids, oleic (18:1), linoleic (C18:2); and linolenic (C18:3) acids. Linolenic acid was most abundant. In dried fronds, all fatty acids decreased at > 35°C. The combined data indicate that the thermotolerance of dry fronds is about 55°C but is at least 10°C lower for hydrated fronds.
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