A new flavanone and a new chalcone, brandisianones F and G, were purified from the roots of Millettia brandisiana Kurz, moreover, sixteen known compounds were found. The chemical structures of all isolated compounds were identified using spectroscopic methods including 1D-NMR, 2D-NMR, MS and IR data. Chalcone 15 exhibited the most cytotoxic activity against liver cancer, HepG2, and cholangiocarcinoma, KKU-M156, cell lines with IC 50 values of 1.74 ± 0.91 and 1.95 ± 0.95 g/mL, respectively. Chalcones 2, 4, 16 and 18 as well as flavones 5, 6 and 12 showed moderate cytotoxicity with IC 50 values ranging from 5.39 to 14.00 g/mL.
A new xanthone derivative, methyl 8-hydroxy-3-hydroxymethylxanthone-1-carboxylate (1), and seven known compounds, 8-hydroxy-3-methylxanthone-1-carboxylate (2), methyl 8-hydroxy-6-methylxanthone-1-carboxylate (3), ergosterol (4), cyathisterone (5), ergosta-4,6,8(14),22-tetraen-3-one (6), calvasterone (7) and 2-hexyl-3-methylmaleic anhydride (8) were first isolated from the fungus Apiospora montagnei. Their structures were elucidated on the basis of spectroscopic analysis (UV, IR, MS, 1D, and 2D NMR). Compounds 5 and 6 showed weak to very weak cytotoxicity against cancer cell lines, NCI-H187 and KB.
The stems of Cryptolepis buchanani are traditionally used for the treatment of inflammation, including arthritis, muscle and joint pain. In the present study, 3 crude extracts (hexane, EtOAc and methanol) from the stems of C. buchanani were evaluated for anti-inflammatory effects using an in vitro model of RAW 264.7 macrophages. At a concentration of 50 μg/mL, the hexane and EtOAc crude extract have inhibited nitric oxide (NO) production by 84.33±2.01 and 82.49±0.92 %, respectively. 3,4-Dihydroxyl benzoic acid (1), vanillic acid (2), syringaldehyde (3), isoscopoletin (4) and stigmast-4-ene-3,6-dione (5) were isolated from hexane and EtOAc crude extracts. Their structures were identified on the basis of 1H and 13C NMR, as well as a comparison of the data from previous reports. Compounds 1-5 were evaluated for anti-inflammatory activity. Compound 3 showed activity of NO inhibition with IC50 values of 49.07 μM (Indomethacin IC50 values of 39.21 μM). In addition, compounds 1-3 and 5 were isolated for the first time from this plant.
HIGHLIGHTS
The crude hexane and ethyl acetate (EtOAc) (each, 50 mg/mL) inhibited nitric oxide (NO) production with inhibition percentage at 84.33 and 82.49, respectively
3,4-Dihydroxyl benzoic acid, vanillic acid, syringaldehyde and stigmast-4-ene-3,6-dione were isolated for the first time from this plant
Syringaldehyde showed activity of NO inhibition with IC50 values of 49.07 μM (Indomethacin IC50 values of 39.21 μM)
GRAPHICAL ABSTRACT
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