Soybean (Glycine max (L.) Merr.) is an important crop for protein, oil, carbohydrates, isoflavones, and many other nutrients to humans and animals. But, antinutritional factors in the raw mature soybean are exist. Raffinose and stachyose are main antinutritional factors in soybean seed. Both raffinose and stachyose are carbohydrates, belonging to the raffinose family of oligosaccharides (RFOs). RFOs are not readily digested in humans and cause flatulence or diarrhea. The objective of this research is to obtain the information on raffinose and stachyose content according to genotype and environment. A total of twenty two soybean genotypes (11 cultivars, 3 germplasms and 8 breeding lines) were selected. Each genotype was grown in the field for two years with two replications and harvested in bulk at natural maturity for two years. Content of raffinose and stachyose was detected by HPLC. The raffinose content (g/kg) of 22 genotypes was 2.68±0.21 -5.87±2.43 in year 1 and was 3.24±0.37 -9.05±0.16 in year 2. The stachyose content (g/kg) was 4.23±0.98 -27.68±9.90 at year 1 and was 5.11±1.09 -25.32±0.35 in year 2. Genotype and environment have highly significant effects on raffinose and stachyose content. Three genotypes (Da-7, 116-13, and RS-78) have low stachyose content at 5% significant level in two years. A positive correlation (R 2 =0.1985*) between raffinose and stachyose was observed in year 2. These informations are valuable in soybean genetics and breeding program related with raffinose and stachyose content.
Keywords
Soybean cultivars with genetically low levels of stachyose enhance the utilization of soybean in food as well as feed uses. The objective of this research is to obtain the information on indirection selection of soybean lines with low stachyose content using DNA marker based on RS2 (rs2) gene. Two genetic populations were developed from the crosses of three parents (116-13 parent : low stachyose content, PI417227 and PI506903 parents: normal stachyose content). Twenty F2 plants of RS2_ genotype and twenty F 2 plants of rs2rs2 genotype from each populations were harvested. Content of stachyose was detected by HPLC. Stachyose contents (g/kg) of 116-13, PI417227, PI506903 parents were 3.7, 23.7, and 17.8, respectively. In population 1, stachyose content 20 F 2 plants with RS2_ genotype was 14.8-24.1 and stachyose content 20 F 2 plants with rs2rs2 genotype was 2.1-4.7. In population 2, stachyose content 20 F 2 plants with RS2_ genotype was 12.4-19.7 and stachyose content 20 F2 plants with rs2rs2 genotype was 2.1-5.0. Mean difference between RS2_ genotype and rs2rs2 genotype in population 1 and 2 was highly significant. From this results, selection of genetic lines with low stachyose content by DNA marker based on RS2 (rs2) gene will be possible.
Abstract. Shear thickening fluid (STF) is a non-Newtonian fluid featuring the increased viscosity upon high strain rate applied. Recently, STF-treated aramid fabrics have been researched to enhance the bulletproof efficiency maintaining the lightweight, however their shear properties including tow shearing, which significantly contribute to the bulletproof properties, have not been characterized, in particular under high shear strain rates. In this study, the shear properties of STF-treated aramid fabrics are characterized using a picture frame test. For this purpose, STF is prepared using polyethylene glycol and silica colloids and coated onto aramid fabrics. Varying the shear strain rate by controlling the pulling speed of the picture frame, the effect of STF on the shear properties of the aramid fabric is investigated. Finally, the shear properties of STF-treated aramid fabrics are predicted a multi-scale energy model and compared with the experiments. This prediction is then extended to cover such a high strain-rate situation as the bullet impacts, enabling to determine the mechanism behind the improved bulletproof performance of the STF-treated fabric.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.