The Swertia chirayita and Artemisia absinthium (Afsanteen) plants extracts were evaluated for phytochemicals and their antimicrobial activity against 3 bacteria and 2 fungal strains by using the disc diffusion technique. Phytochemical analysis showed the presence of tannins, flavonoids, saponins, and terpenoids in Swertia chirayita while in Artemisia absinthium only saponins, terpenoids were present. To a very small extent tannins were present in the ethanolic extract of Afsanteen. But both plant extracts gave negative results for the presence of cardiac glycosides. Antibacterial activity of Swertia chirayita and Afsanteen plant extracts were screened against 3 bacterial strains (E. coli, Bacillus subtilis, Pseudomonas syringola) and 2 fungal strains (Aspergillus niger and Fusarium Solani). Ampicillin was used as a standard drug for antibacterial and antifungal activity. Results showed that Afsanteen and chirayita extracts showed activity against bacterial strains except for chirayita n-hexane and ethanol extract for Bacillus subtilis and Chirayita n-hexane extract for Pseudomonas syringola. A maximum zone of inhibition was noticed for chirayita ethanol extract against Pseudomonas syringola. But both plant extracts showed zero activity against fungal strains except Chirayita acetone extract against Fusarium solani while chirayita ethanol extract against Aspergillus niger. Both extracts of plants gave concentration-dependent activity. It was concluded that the presence of antimicrobial activity for both plant extracts indicated that is due to the presence of phytochemical compounds.
Introduction: The current research was performed to evaluate antimicrobial, anti-fungal and biochemical properties of Linum usitatissimum (flax seeds) and Syzygium aromaticum (clove). Flaxseeds is one of dietary sources containing phenolics, named lignans. Syzygium aromaticum ordinarily called clove is generally considered second important flavor in world and is broadly developed in North Maluku Islands in Indonesia. Glycosides, saponins, flavonoids, steroids, tannins, alkaloids, terpenes, and other phytochemical constituents have been shown to have pharmacological actions in different activities. Syzygium aromaticum (clove) and Linum usitatissimum (flax) have unique pharmacological effects and have been utilized for food safety.Materials and Methods: In this study, ethanolic, methanolic and aqueous extracts of Linum usitatissimum and Syzygium aromaticum were evaluated against the 3 bacterial strains and 2 contagious fungal strains viz. A. niger and F. solani.Results: Among the bacterial strains i.e., E. coli, P. syringae and B. subtilis, antimicrobial action was noted by Syzygium aromaticum against E. coli, methanolic and aqueous extract of Linum usitatissimum and ethanolic extract of S. aromaticum showed antimicrobial action against the P. syringae and B. subtilis. Best antimicrobial action was observed by the ethanolic extract of the L. usitatissimum and methanolic and aqueous extract of S. aromaticum. Regarding parasitic strains of A. niger and F. solani, 3 extracts of L. usitatissimum showed satisfactory results against A. niger strain and 2 extracts (ethanolic and methanolic) of S. aromaticum against F. solani strain, whereas aqueous extracts of both the plants had no effect against F. solani strain. The phytochemical screening showed presence of terpenoids, tannins, flavonoids, saponins and cardiovascular glycosides in both plants.Conclusion: Flaxseed and clove extracts were found potent antimicrobial agents.
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