Five normal placentae of normal pregnancy and delivery were used to study the gross morphology and ultrastructure of the dendritic cells in the normal human decidua. Zinc iodide osmium (ZIO) mixture was prepared. Small pieces of the placenta were processed for light microscopy and electron microscopy. For light microscopy, the small pieces of placenta were incubated in 20 mM PBS-EDTA solution, ph 7.4 at 37 degrees C to detach the basal plate. The basal plate pieces were incubated in ZIO. A wholemount preparation of the basal plate demonstrated the whole profile and gross morphology of the dendritic cell. For electron microscopy, the placenta pieces were fixed in 3% glutaraldehyde in 0.1 M phosphate buffer, ph 7.4, washed with phosphate buffer, put in ZIO mixture, washed in distilled water, dehydrated in graded ethanol, cleared in propylene oxide, and embedded in resin. Ultra thin sections of the ZIO blocks were cut using a diamond knife and stained with lead citrate. Ultrastructure of the dendritic cell presented multiple cytoplasmic processes, lobulated or round or oval, heterochromatic or euchromatic nucleus, mitochondria, free ribosomes, and pieces of rough endoplasmic reticulum, but no Birbeck granules.
Aims To compare morning and evening salivary melatonin levels, sleep quality and chronotype between nurses working fixed day shifts and those working rotating night shifts. Background Rotating night shift work is an inevitable part of nursing and is a major reason for disrupted sleep. Design Cross‐sectional comparative design. Participants and Settings We used cluster sampling to recruit 520 female nurses working fixed day and rotating night shifts in the United Arab Emirates. Methods Morning and evening melatonin were measured from corresponding saliva samples. The Pittsburgh Sleep Quality Index used to evaluate sleep quality and self‐assessment of preferred circadian times was used to assess participants’ chronotypes. Data were collected between October 2017–December 2018. Results Rotating night shift nurses had significantly lower evening melatonin compared with the fixed day shift group. No significant difference was found in sleep quality between the groups, although more participants in the rotating night shift group (N = 110, 42.31%) expressed better sleep quality than those in the fixed day group (N = 90, 36.00). Participants in the rotating night shift group expressed better subjective sleep, longer sleep duration, less disturbed sleep and better daily function (p > .05) than the fixed day group. Rotating night shift participants were more likely to have evening or intermediate chronotypes and more likely to report alignment of shift work to their chronotype compared with fixed day shift participants (p = .001). Longer shift duration, marital status and city location were associated with reduced evening melatonin among nurses on rotating night shifts. Conclusion Rotating night shift nurses showed evidence of better sleep than those from the fixed day shift. Proper shift assignment, chronotype and alignment with shift work appeared to produce better sleep quality. Impact Organizational policy must consider a novel model for work schedules that allow adequate off‐duty days for sleep recovery among nurses.
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