Gross morphology and ultrastructure of dendritic cells in the normal human decidua

Abraham, Suma; Indrasingh, Inbam; Vettivel, Selvakumar; Chandi, G.

doi:10.1002/(sici)1098-2353(2000)13:3<177::aid-ca3>3.0.co;2-7

Cited by 12 publications

(4 citation statements)

References 11 publications

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“…Careful comparison with bona fide DCs obtained in vitro from peripheral blood mononuclear cells treated with GM‐CSF and IL‐4 [34], as well as with unequivocal lamina propria DCs, supported this conclusion. This was also confirmed by the reactivity of such intraepithelial cells, and especially their cytoplasmic vesicles, with antibodies directed against DC‐SIGN, a Ca 2+ ‐binding C‐type lectin known to be a marker of immature DCs and to selectively interact with H. pylori [2,27,28,43], as well as with the ZIO technique, a well‐known marker of both Langerhans cells, with special reference to Birbeck bodies [29,38], and DCs [30,44,45]. Given that C‐type lectins are important components of both Langerhans cell granules [46] and DC vesicles, that Zn complexes form a relevant part of electron dense ZIO precipitates [47], and that CaI 2 can be substituted for ZnI 2 in the ZIO reactive with equal results [48], a possible contribution of C‐type lectins to the ZIO reactivity of Langerhans and dendritic cells should be considered.…”

Section: Discussionmentioning

confidence: 94%

Evidence for Transepithelial Dendritic Cells in Human H. pylori Active Gastritis

Necchi¹,

Manca

Ricci

et al. 2009

Helicobacter

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Background: Despite extensive experimental investigation stressing the importance of bacterial interaction with dendritic cells (DCs), evidence regarding direct interaction of Helicobacter pylori or its virulence products with DCs in the human gastric mucosa is lacking. Methods: Human gastric mucosa biopsies, with or without H. pylori infection and active inflammation, were investigated at light and electron microscopy level with immunocytochemical tests for bacterial products (VacA, urease, outer membrane proteins) and DC markers (DC-SIGN, CD11c, CD83) or with the DC-labeling ZnI 2 -OsO 4 technique. Parallel tests with cultured DCs were carried out. Results: Cells reproducing ultrastructural and cytochemical patterns of DCs were detected in the lamina propria and epithelium of heavily infected and inflamed (but not of normal) mucosa, where DC luminal endings directly contact H. pylori and take up their virulence products. Cytotoxic changes (mitochondrial swelling, cytoplasmic vacuolation, autophagy) were observed in intraepithelial DCs and reproduced in cultured DCs incubated with H. pylori broth culture filtrates to obtain intracellular accumulation of VacA and urease. Granulocytes were also seen to contact and heavily phagocytose luminal H. pylori , while macrophages remained confined to basal epithelium, though taking up bacteria and bacterial products. Conclusion: Human DCs can enter H. pylori -infected gastric epithelium, in association with other innate immunity cells, to take up bacteria and their virulence products. This process is likely to be important for bacterial sensing and pertinent immune response; however, it may also generate DC cytotoxic changes potentially hampering their function.

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Section: Discussionmentioning

confidence: 94%

Evidence for Transepithelial Dendritic Cells in Human H. pylori Active Gastritis

Necchi¹,

Manca

Ricci

et al. 2009

Helicobacter

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“…Morphologic characteristics of these cells were similar to those of normal histiocytes and of other canine HS cell lines established previously (Table 1). 1,3,4,12,22,31…”

Section: Discussionmentioning

confidence: 99%

Variations in Histiocytic Differentiation of Cell Lines From Canine Cerebral and Articular Histiocytic Sarcomas

et al. 2017

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Two newly established canine histiocytic sarcoma (HS) cell lines, designated as PWC-HS01 and FCR-HS02, were obtained from brain and articular tumors, respectively. These 2 HS cell lines had phagocytic ability and modal chromosome aberrations. Although morphologic features of both HS cells were similar, immunocytochemical examinations revealed that the PWC-HS01 cell line expressed both dendritic cell (ie, S100, CD208, CD1, and CD4) and macrophage (ie, CD68, CD163, and CD204) markers. In contrast, the FCR-HS02 cell line was immunonegative for CD204 and CD68 but consistently positive for the dendritic cell markers. Moreover, reverse transcription polymerase chain reaction analyses confirmed histiocytic differentiation of both HS cell lines. These results suggest that HS from the central nervous system may have a tendency to be more undifferentiated compared with cases from other organs. In addition, the 2 newly established HS cell lines were also tumorigenic and metastatic in immunodeficient mice, supporting that these cell lines can be used as new tumor models for investigating canine histiocytic diseases.

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“…Using electron microscopy, DCs were characterized in human decidua as: containing multiple cytoplasmic lobulated processes, being either round or oval with a heterochromatic or eucromatic nucleus and free of Birbeck granules (Abraham, 2000), the latter feature clearly separating them from classical Langerhans cells. In addition, it appears to be clear that human decidual DC belong predominantly to the myeloid lineage since CD123, characteristic for plasmacytoid DC, could not (Gardner, 2003) or only sparsely (Miyazaki, 2003) be detected on decidual cells.…”

Section: Dendritic Cells At the Uterine Mucosa Deciduamentioning

confidence: 99%

Role of Dendritic Cells in the Regulation of Maternal Immune Responses to the Fetus During Mammalian Gestation

Kämmerer

Kruse²,

Barrientos³

et al. 2008

LIMM

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Successful mammalian pregnancy relies on the action of sophisticated regulatory mechanisms that allow the fetus (a semi-allograft) to grow and develop in the uterus in spite of being recognized by maternal immune cells. Among several immunocompetent cells present at the maternal fetal interface, dendritic cells (DC) seem to be of particular relevance for pregnancy maintenance given their unique ability to induce both antigen-specific immunity and tolerance. Thus, these cells would be potentially suitable candidates for the regulation of local immune responses within the uterus necessary to meet the difficult task of protecting the mother from infection without compromising fetal survival. Current evidence on decidual DC phenotype and function, and their role in the regulation of the maternal immune system during mouse and human pregnancy are discussed and reviewed herein; highlighting novel DC functions that seem to be of great importance for a successful pregnancy outcome.

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Gross morphology and ultrastructure of dendritic cells in the normal human decidua

Cited by 12 publications

References 11 publications

Evidence for Transepithelial Dendritic Cells in Human H. pylori Active Gastritis

Evidence for Transepithelial Dendritic Cells in Human H. pylori Active Gastritis

Variations in Histiocytic Differentiation of Cell Lines From Canine Cerebral and Articular Histiocytic Sarcomas

Role of Dendritic Cells in the Regulation of Maternal Immune Responses to the Fetus During Mammalian Gestation

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