Andrographolide isolated from Andrographis paniculata Ness (Acanthaceae) at 0.35 mM , 0.70 mM and 1.40 mM induced DNA fragmentation and increased the percentage of apoptotic cells when TD-47 human breast cancer cell line was treated for 24 , 48 and 72 h. The results demonstrated that andrographolide can induce apoptosis in TD-47 human breast cancer cell line in a time and concentration-dependent manner by increase expression of p53, bax , caspase-3 and decrease expression of bcl-2 determined by immunohistochemical analysis.