Apoptosis Inducing Effect Of Andrographolide On TF-47 Human Breast Cancer Cell Line

Abstract

Andrographolide isolated from Andrographis paniculata Ness (Acanthaceae) at 0.35 mM , 0.70 mM and 1.40 mM induced DNA fragmentation and increased the percentage of apoptotic cells when TD-47 human breast cancer cell line was treated for 24 , 48 and 72 h. The results demonstrated that andrographolide can induce apoptosis in TD-47 human breast cancer cell line in a time and concentration-dependent manner by increase expression of p53, bax , caspase-3 and decrease expression of bcl-2 determined by immunohistochemical analysis.

The Protective Effect of the Ethanol Leaf Extract of Andrographis Paniculata on Cisplatin-Induced Acute Kidney Injury in Rats Through nrf2/KIM-1 Signalling Pathway

“…Plants with diverse medicinal properties especially those rich in anti-oxidant, anticarcinogenic and probably anti-inflammatory effects have come under much research activities and Andrographis paniculata (AP) is one of such [11,12]. AP has been reported to have a broad range of pharmacological effects including antioxidant [13,14], hepatoprotective [15], anticancer [16,17] anti-inflammatory [18], cardioprotective [19], antidiarrheal [20], antihyperglycemic [21], antihepatitis [22], anti-HIV [23], antimicrobial, antimalarial [24], cytotoxic [23] and immunostimulatory [25]. Several phytochemicals including 55 ent-labdane diterpenoids, 30 flavonoids, 8 quinic acids, 4 xanthones, andrographidoids A, B, C, D, and E as well as reported 3 new ent-labdane diterpenoids, namely, 19norandrographolides A, B, and C have been reported in the plant [26][27][28].…”

Chemopeventive Effects of Andographis Panniculata Extract in Vivo and in Vitro Models

“…Andrographolide, a Andrographis panniculata compound was also reportedly induced apoptosis in a TD-47 human breast cancer cell line in a time and concentrationdependent manner. It also increased expression of p53, bax and caspase-3 and decreased bcl-2 expression as shown by immunohistochemical analysis was observed [8] . The anticarcinogenic activity of andrographis panniculata in skin papilloma model in Swiss albino mice was observed.…”

“…There are few reports on the cytotoxic and antiproliferative effects of Andrographis. paniculata up on in vitro cell lines [8,13,14] . Andrographolide, a Andrographis panniculata compound was also reportedly induced apoptosis in a TD-47 human breast cancer cell line in a time and concentrationdependent manner.…”

“…Andrographis paniculata antioxidant and hepatoprotective effect on acetaminophen (Paracetamol) induced hepatotoxicity in albino rats are also reported [7] . An andrographolide was reported to induce apoptosis in TD-47 human breast cancer cell line in a time and concentrationdependent manner by increase expression of p53, bax, and caspase-3 and decrease expression of bcl-2 determined by immune histochemical analysis [8] . Andrographis paniculata, dry leaf powder when fed orally to male albino rats, at a dose level of 20 mg per day for 60 days was reported to spermatogenesis cessation, cessation of degenerative changes in the seminiferous tubules, Leydig cells regression and regressive and/or degenerative changes in the epididymis, seminal vesicle, ventral prostate and coagulating gland [9] .…”

Pharmacokinetic and Pharmacodynamic Interaction of Andrographolide and Standardized Extract of Andrographis paniculata (Nees) with Nabumetone in Wistar Rats

“…The hepatoprotective activity of AP and AN has been previously reported and scientifically proven (Visen et al ., ; Chua, ). AP and AN have several other biological activities including antidiabetic (Nugroho et al ., ), anticancer (Harjotaruno et al ., ) and antiinflammatory properties (Hidalgo et al ., ; Parichatikanond et al ., ).…”