Abstract. Rahayuwati S, Kusumah YM, Prawirosukarto S, Dadang, Santoso T. 2020. Genetic variability of Indonesian Oryctes rhinoceros nudivirus (OrNV) as genus of Alphanudivirus. Biodiversitas 21: 2047-2055. Oryctes rhinoceros nudivirus (OrNV) is known as a successful classical biological control agent. Recent reports have revealed differences in pathogenicity among OrNV isolates, with correlation suspected between virus virulence and genetic variability. The aim of the research is to study the genetic variability of OrNV isolates based on the DNA polymerase (DNA pol) gene and their phylogenetic relationship with Genus Alphanudivirus. Oryctes rhinoceros (Coleoptera: Scarabaeidae) as an OrNV host were collected from Sumatra, Belitung, Java, Kalimantan, and Sulawesi. Primers were designed to amplify complete gene of DNA pol. Sequencing, phylogenetic, and investigate genetic diversity scores were used to determine variability at nucleotide level. Five primers successfully amplified the entire DNA pol gene. Genetic variability of OrNV was high, from nine isolates found eight genetically different. Isolates could be divided into two groups of genetic diversity: high and low. High genetic diversity of OrNV supposed as an old population that occupied Sundaland and Wallacean paleogeography at first appearance in Miocene Era until early Pliocene 5 Mya. New population of OrNV had low genetic diversity were travel cross-island that supposed to correlate with oil palm plantation development and the genetic drift. The benefit information of the OrNV genetic variability is as preliminary guideline for virulence test. OrNV is a member of Nudiviridae family, Genus Alphanudivirus, and at recent moment they consist of eight species. Alphanudivirus infect Coleoptera, Diptera, Hemiptera and Orthoptera order that soil as immature niche may explain their closely related taxa.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.