No abstract

Fischer carbene complex anchored on glass or silicon surface using a Cu-free 'click' reaction allows facile and swift covalent grafting of protein molecules like Bovine Serum Albumin (BSA).

A Fischer carbene complex was grafted onto self-assembled monolayers (SAMs) on gold or glass by a copper-free "click" reaction. Pendant lysine residues of protein A obtained from Staphylococcus aureus rapidly reacted with the electrophilic metal complex on SAM effecting a covalent attachment of protein A with the surface. The protein A coated surface further led to bioaffinity immobilization of rabbit IgG in an oriented manner, a feature that also permits its purification from rabbit serum. Rabbit IgG could be removed from protein A coated surface by pH adjustment. The regenerated protein A surface was reused three times without loss of activity.

An efficient synthesis of Pd nanoparticles in water has been developed using a Fischer carbene complex of tungsten as the reductant and PEG as the capping agent. The colloidal palladium (1 mol %) efficiently catalyzes Hiyama cross-coupling reactions performed in air. Excellent yields of products were obtained with a wide range of substrates. Catalytic activity and stability of the nanoparticles were found to be inversely correlated.

This paper compares the DNA interactions of a bis 9‐aminomethylanthracene dye (2) vs. a mono‐anthracene (4) under high ionic strength conditions similar to those in the cell nucleus (∼150 mM NaCl and 260 mM KCl). The chloride salts triggered an enhancement in anthracene‐sensitized DNA photocleavage, where bis‐anthracene 2 exhibited superior DNA binding affinity, faster reaction kinetics, and higher levels of DNA damage at low‐ to sub‐micro molar dye concentrations (350 nm hν, pH 7.0). While spectroscopic and viscometric data suggested that the chloride salts reduced DNA intercalation in favor of a non‐intercalative binding mode in which 2 and 4 were better able to generate hydroxyl radicals, there was evidence of persistent intercalation of the second anthracene ring of 2. We propose that such an interaction anchored the bis‐anthracene to DNA under the high ionic strength conditions, increasing its binding affinity and photocleaving capabilities relative to the mono analog (4).